Pair-rule expression of the Drosophila fushi tarazu gene: a nuclear receptor response element mediates the opposing regulatory effects of runt and hairy
| Autor: | Chihcheng Tsai, P. Gergen |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Genotype
Response element Molecular Sequence Data Fushi Tarazu Transcription Factors Genes Insect Biology Genes Reporter Transcriptional regulation Basic Helix-Loop-Helix Transcription Factors Morphogenesis Animals Drosophila Proteins Promoter Regions Genetic Molecular Biology Gene In Situ Hybridization Genetics Homeodomain Proteins Reporter gene Base Sequence Runt Drosophila embryogenesis Gene Expression Regulation Developmental Nuclear Proteins DNA-Binding Proteins Repressor Proteins Blastocyst Nuclear receptor Insect Hormones Mutation Insect Proteins Drosophila Blastoderm Developmental Biology Transcription Factors |
| Zdroj: | Development (Cambridge, England). 121(2) |
| ISSN: | 0950-1991 |
| Popis: | The segmentation genes runt and hairy are required for the proper transcriptional regulation of the pair-rule gene fushi tarazu during the blastoderm stage of Drosophila embryogenesis. The expression of different fushi tarazu reporter genes was examined in runt and hairy mutant embryos, as well as in runt over-expressing embryos in order to identify DNA elements responsible for mediating these regulatory effects. The results indicated that runt and hairy act through a common 32 base-pair element. This element, designated as fDE1, contains a binding site for a small family of orphan nuclear receptor proteins that are uniformly expressed in blastoderm embryos. The pair-rule expression of reporter gene constructs containing multi-merized fDE1 elements depends on activation by runt and repression by hairy. Examination of reporter genes with mutated fDE1 elements provided further evidence that this element mediates both transcriptional activation and repression. Genetic experiments indicated that the opposing effects of runt and hairy were not due solely to cross-regulatory interactions between these two genes and that fDE1-dependent expression is regulated by factors in addition to runt and hairy. |
| Databáze: | OpenAIRE |
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