Early stage of marine biofilm formation on duplex stainless steel

Autor: Antoine Seyeux, Philippe Marcus, Elise Gardin, Audrey Allion-Maurer, Sandrine Zanna, Dimitri Mercier
Přispěvatelé: Institut de Recherche de Chimie Paris (IRCP), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Ministère de la Culture (MC), APERAM
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Scanning electron microscope
Surface Properties
General Physics and Astronomy
Artificial seawater
chemistry.chemical_element
Spectrometry
Mass
Secondary Ion
02 engineering and technology
010402 general chemistry
01 natural sciences
General Biochemistry
Genetics and Molecular Biology
Bacterial Adhesion
Biomaterials
Chromium
Pseudoalteromonas
X-ray photoelectron spectroscopy
Fluorescence microscope
General Materials Science
Seawater
14. Life underwater
ComputingMilieux_MISCELLANEOUS
biology
Chemistry
Biofilm
General Chemistry
[CHIM.MATE]Chemical Sciences/Material chemistry
021001 nanoscience & nanotechnology
biology.organism_classification
Stainless Steel
0104 chemical sciences
Secondary ion mass spectrometry
Chemical engineering
Microscopy
Fluorescence
Biofilms
Microscopy
Electron
Scanning
0210 nano-technology
Zdroj: Biointerphases
Biointerphases, American Vacuum Society, 2020, 15 (4), pp.041014. ⟨10.1116/6.0000361⟩
ISSN: 1559-4106
DOI: 10.1116/6.0000361⟩
Popis: The aim of this work was to investigate the bacteria-surface interactions occurring during the first hour of adhesion of marine Pseudoalteromonas NCIMB 2021 at the surface of 2304 lean duplex stainless steel in artificial seawater. A complete characterization of the biofilm and the passive film was performed coupling epifluorescence microscopy, scanning electron microscopy (SEM), x-ray photoelectron spectroscopy (XPS), and time of flight secondary ion mass spectrometry (ToF-SIMS). The coupling of XPS and ToF-SIMS analyses revealed that (1) protein and polysaccharide contents in the biofilm are similar in the presence or absence of nutrients, (2) the biofilm is mainly composed of proteins and the protein content is similar to the one of Tightly Bound EPS, (3) increased bacterial activity due to nutrients leads to chromium enrichment in the passive film in close vicinity to the bacteria.
Databáze: OpenAIRE
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