Therminator DNA Polymerase: Modified Nucleotides and Unnatural Substrates
| Autor: | William E. Jack, Madeleine M. Boyle, Jackson Buss, Alexandra M. Gehring, Vladimir Potapov, Andrew F. Gardner, Kelly M Zatopek, Jennifer L. Ong, Kiserian M. Jackson, Ivan R. Corrêa |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
DNA polymerase Aptamer aptamers Review Computational biology Biochemistry Genetics and Molecular Biology (miscellaneous) Biochemistry functionalized DNA DNA sequencing 03 medical and health sciences Synthetic biology xenonucleic acids 0302 clinical medicine Molecular Biosciences Nucleotide lcsh:QH301-705.5 Molecular Biology chemistry.chemical_classification unnatural base pairs biology 030104 developmental biology lcsh:Biology (General) chemistry 030220 oncology & carcinogenesis GenBank biology.protein |
| Zdroj: | Frontiers in Molecular Biosciences, Vol 6 (2019) Frontiers in Molecular Biosciences |
| ISSN: | 2296-889X |
| Popis: | A variant of 9°N DNA polymerase [Genbank ID (AAA88769.1)] with three mutations (D141A, E143A, A485L) and commercialized under the name “Therminator DNA polymerase” has the ability to incorporate a variety of modified nucleotide classes. This Review focuses on how Therminator DNA Polymerase has enabled new technologies in synthetic biology and DNA sequencing. In addition, we discuss mechanisms for increased modified nucleotide incorporation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|