Cloning and expression of a second Aspergillus niger pectin lyase gene (pelA): Indications of a pectin lyase gene family in A. niger
| Autor: | Jaap Visser, M. A. Kusters Van Someren, J.A.M. Harmsen |
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| Rok vydání: | 1990 |
| Předmět: |
Blotting
Western Molecular Sequence Data Restriction Mapping Heterologous hybridization Molecular cloning Laboratorium voor Erfelijkheidsleer Transformation Genetic Pectin lyase Gene Expression Regulation Fungal Gene expression Genetics Gene family Genomic library Amino Acid Sequence Cloning Molecular Pectinase pel genes Gene Polysaccharide-Lyases Base Sequence biology Aspergillus niger General Medicine biology.organism_classification Molecular biology Biochemistry Multigene Family Laboratory of Genetics Plasmids |
| Zdroj: | Current Genetics, 18, 161-166 Current Genetics 18 (1990) |
| ISSN: | 1432-0983 0172-8083 |
| DOI: | 10.1007/bf00312604 |
| Popis: | Using the previously cloned Aspergillus niger N756 pectin lyase D gene as a probe, the corresponding pelD gene has been isolated from a genomic library of the loboratory strain A. niger N400. This gene encodes PLD, previously described as PLI, which is one of the two major pectin lyases isolated from the commeriial pectinase preparation Ultrazym®. Heterologous hybridization of the A. niger N400 genomic library with the pelD gene led to the isolation of another five genes: pelA, B, C, E, and F. These genes differ in their hybridization patterns with probes containing either the entire pelD gene, or 5′ or 3′ parts thereof. By partial sequencing, and expression in an A. niger transformant containing multiple copies of the pelA gene, we show that this gene, which hybridizes strongest with the pelD gene, encodes the other major pectin lyase from Ultrazym®, PLII. |
| Databáze: | OpenAIRE |
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