Three-Stranded Clip of the Oligonucleotide 5′-(dT)10pO(CH2CH2O)3p(dT)10pO(CH2CH2O)3p(dA)10-3′
| Autor: | Il'icheva Ia, Y.P. Lysov, V. L. Florentiev, Anna K. Shchyolkina, Olga F. Borisova, Edward N. Timofeev, O.K. Mamayeva, B.P. Gottikh |
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| Rok vydání: | 1994 |
| Předmět: |
Molecular Sequence Data
Intercalation (chemistry) Complex formation chemistry.chemical_compound Genetics Molecule Nucleotide Chromatography High Pressure Liquid chemistry.chemical_classification Base Composition Binding Sites Base Sequence Oligonucleotide Circular Dichroism Phosphate buffered saline Temperature DNA Hydrogen-Ion Concentration Crystallography Spectrometry Fluorescence Oligodeoxyribonucleotides chemistry Biochemistry Nucleic Acid Conformation Thermodynamics Spectrophotometry Ultraviolet Ethidium bromide Triple helix |
| Zdroj: | Antisense Research and Development. 4:27-33 |
| ISSN: | 1050-5261 |
| DOI: | 10.1089/ard.1994.4.27 |
| Popis: | Temperature dependence of UV and CD spectra of the oligonucleotide 5'-(dT)10-L-(dT)10-L-(dA)10-3' [tripl(ATT)] [L = -pO(CH2CH2O)3p-] in phosphate buffer, pH 7, at various NaCl concentrations and in the presence or absence of 0.01 M MgCl2 has been studied. At low oligonucleotide concentrations (2.2 x 10(-5) M nucleotide concentration) all structural transitions proceed intramolecularly. Tripl(ATT) exists in three forms: as a three-stranded clip (at low temperatures), a double-stranded hairpin (at intermediate temperatures), and as an open strand (at high temperatures). Thermodynamic parameters of the triplex formation depending on the NaCl concentration were calculated. The CD spectra were assigned to the single-, double-, and three-stranded forms. Ethidium bromide (EtBr) binding to the three-stranded clip was studied. Ethidium bromide molecules were shown to intercalate into the triple helix with the stable complex formation (association constant is 10(6)). One molecule of three-stranded clip binds not more than three EtBr molecules. The proposed synthetic model (oligonucleotide blocks coupled by hydroxyalkyl chains) has been shown to be convenient for studies of the physical and chemical properties of the triplex and other multistranded complexes of DNA. |
| Databáze: | OpenAIRE |
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