Tandem mass tag-based quantitative proteomic profiling of the serum of patients with abnormal uterine bleeding associated with copper intrauterine device
| Autor: | Jing Du, Junjie Sun, Linfen Xu, Yan Che, Xiaoli Liu, Xiumei Xiong, Yupei Shen, Jianhua Xu, Weiqiang Zhu, Zhaofeng Zhang, Lifang Jiang, Xiujuan Chen, Yanyan Mao, Junwei Liu, Fang Liu, Xiaoqin Yan |
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| Rok vydání: | 2020 |
| Předmět: |
Adult
Proteomics Quantitative proteomics Tandem mass tag Intrauterine device Pathogenesis Andrology 03 medical and health sciences Young Adult 0302 clinical medicine Tandem Mass Spectrometry Medicine Humans 030212 general & internal medicine 030219 obstetrics & reproductive medicine Proteomic Profiling business.industry Area under the curve Obstetrics and Gynecology General Medicine Intrauterine Devices Copper Biomarker (medicine) Female Uterine Hemorrhage business Chromatography Liquid |
| Zdroj: | International journal of gynaecology and obstetrics: the official organ of the International Federation of Gynaecology and ObstetricsREFERENCES. 154(1) |
| ISSN: | 1879-3479 |
| Popis: | OBJECTIVE To investigate changes in the level of protein in serum and uncover the underlying pathogenesis of abnormal uterine bleeding (AUB) associated with copper intrauterine devices (Cu IUD). METHODS Protein profiles were investigated via tandem mass tag (TMT)-based quantitative proteomics and bioinformatics technology. Quantification and characterization of candidate proteins were further performed in 33 controls and 45 cases by Luminex assay and enzyme-linked immunosorbent assay. RESULTS In total, 842 proteins were identified via TMT coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) in the serum of individuals with IUDs. Among them, 25 differentially expressed proteins (p |
| Databáze: | OpenAIRE |
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