Reprogramming bacterial protein organelles as a nanoreactor for hydrogen production
| Autor: | Qiang Wang, Jiafeng Huang, Reiner Sebastian Sprick, Andrew I. Cooper, Hai Lun He, Tianpei Li, Catherine M. Aitchison, Lu-Ning Liu, Mengru Yang, Gregory F. Dykes, Qiuyao Jiang, Fang Huang |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Iron-Sulfur Proteins Hydrogenase Ribulose-Bisphosphate Carboxylase Science General Physics and Astronomy Bioengineering Nanoreactor 010402 general chemistry Biosynthesis 01 natural sciences General Biochemistry Genetics and Molecular Biology Article Catalysis 03 medical and health sciences Synthetic biology Bioreactors Bacterial Proteins Nanocapsules Organelle Escherichia coli QD Bioenergy Photosynthesis lcsh:Science Hydrogen production Organelles Multidisciplinary Chemistry Rational design General Chemistry 0104 chemical sciences Halothiobacillus Carboxysome 030104 developmental biology Genes Bacterial Biophysics Biocatalysis lcsh:Q Hydrogen |
| Zdroj: | Nature Communications Nature Communications, Vol 11, Iss 1, Pp 1-10 (2020) |
| ISSN: | 2041-1723 |
| Popis: | Compartmentalization is a ubiquitous building principle in cells, which permits segregation of biological elements and reactions. The carboxysome is a specialized bacterial organelle that encapsulates enzymes into a virus-like protein shell and plays essential roles in photosynthetic carbon fixation. The naturally designed architecture, semi-permeability, and catalytic improvement of carboxysomes have inspired rational design and engineering of new nanomaterials to incorporate desired enzymes into the protein shell for enhanced catalytic performance. Here, we build large, intact carboxysome shells (over 90 nm in diameter) in the industrial microorganism Escherichia coli by expressing a set of carboxysome protein-encoding genes. We develop strategies for enzyme activation, shell self-assembly, and cargo encapsulation to construct a robust nanoreactor that incorporates catalytically active [FeFe]-hydrogenases and functional partners within the empty shell for the production of hydrogen. We show that shell encapsulation and the internal microenvironment of the new catalyst facilitate hydrogen production of the encapsulated oxygen-sensitive hydrogenases. The study provides insights into the assembly and formation of carboxysomes and paves the way for engineering carboxysome shell-based nanoreactors to recruit specific enzymes for diverse catalytic reactions. The extreme oxygen sensitive character of hydrogenases is a longstanding issue for hydrogen production in bacteria. Here, the authors build carboxysome shells in E. coli and incorporate catalytically active hydrogenases and functional partners within the empty shell for the production of hydrogen. |
| Databáze: | OpenAIRE |
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