Proteomics for the diagnosis of thyroid lesions: Preliminary report
| Autor: | Vittorio Giardini, Manuel Galli, Marcella Scardilli, A. Vanzati, Francesca Bono, G. De Sio, Andrew Smith, Veronica Mainini, Fabio Pagni, Paolo Goffredo, Mattia Garancini, Fulvio Magni |
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| Přispěvatelé: | Pagni, F, Mainini, V, Garancini, M, Bono, F, Vanzati, A, Giardini, V, Scardilli, M, Goffredo, P, Smith, A, Galli, M, DE SIO, G, Magni, F |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Thyroid nodules
Adult Male Proteomics Pathology medicine.medical_specialty Histology Biopsy Fine-Needle Thyroid Gland Thyroid cancer Pathology and Forensic Medicine Thyroid carcinoma In vivo medicine Humans Thyroid Neoplasms Biomarker discovery Aged Aged 80 and over Proteomic Profile medicine.diagnostic_test business.industry Thyroid Carcinoma General Medicine Middle Aged medicine.disease Carcinoma Papillary Carcinoma Neuroendocrine Fine-needle aspiration medicine.anatomical_structure Medullary carcinoma Thyroid Cancer Papillary Carcinoma Medullary Female business Biomarkers |
| Popis: | Objective Matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS) is a unique proteomic technology that explores the spatial distribution of biomolecules directly in situ, thus integrating molecular and morphological information. The possibility of correlating distribution maps of multiple analyses with cytological features makes it an ideal research tool for discovering new diagnostic markers. A previous study showed that MALDI-IMS could help discrimination between different types of thyroid lesions, especially papillary thyroid carcinoma (PTC); the present feasibility study on ex vivo fine needle aspiration (FNA) smears describes its potential in detecting new proteomic targets of other thyroid lesions (follicular lesions, medullary carcinoma). Methods MALDI-IMS was conducted on ex vivo FNAs obtained from surgical specimens and corresponding in vivo samples. Differences between proteomic profiles of different thyroid lesions were compared. Results Comparing the protein profiles of hyperplastic nodules obtained from three different patients with each other, and with a new PTC, showed a high degree of concordance, indicating good reproducibility of the IMS technology on cytological samples, suggesting its potential as a tool for biomarker discovery. Furthermore, comparison of the average proteomic profiles of hyperplastic nodules with a Hurthle cell adenoma revealed significant differences, underlying the capability of MALDI-IMS to distinguish between different thyroid lesions. Finally, the proteomic profile of medullary thyroid carcinoma was also characterized. Conclusions Our results confirmed the possible role of MALDI-IMS in the search for diagnostic targets of PTC and follicular lesions, which could be applied in larger trials aimed at the identification of proteins, convertible to cost-effective diagnostic tools such as immunohistochemistry. These tests could be used to analyse in vivo cytological smears, improving the preoperative diagnosis of indeterminate thyroid nodules. |
| Databáze: | OpenAIRE |
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