In-gel detection of L-amino acid oxidases based on the visualisation of hydrogen peroxide production
| Autor: | Jan Erik Rau, Ulrich Fischer |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Microbiology (medical)
chemistry.chemical_classification Oxidase test Molecular mass Bacteria Chemistry Hydrogen Peroxide L-amino-acid oxidase L-Amino Acid Oxidase Microbiology Combinatorial chemistry Amino acid chemistry.chemical_compound Enzyme Screening method Organic chemistry Electrophoresis Polyacrylamide Gel Amino Acids Hydrogen peroxide Molecular Biology Oxidation-Reduction |
| Zdroj: | Journal of microbiological methods. 85(3) |
| ISSN: | 1872-8359 |
| Popis: | A simple and practicable method for in-gel detection of bacterial produced L-amino acid oxidases (L-AAOs) after non-denaturing SDS-PAGE based on the visualisation of occurring hydrogen peroxide production is described. Advantages of this screening method for L-AAOs are the determination of their numbers and approximate molecular weights additionally in one approach. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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