Rapid Detection of Salmonella enterica via Directional Emission from Carbohydrate-Functionalized Dynamic Double Emulsions
| Autor: | Sara Nagelberg, Lukas Zeininger, Timothy M. Swager, Suchol Savagatrup, Kosuke Yoshinaga, Mathias Kolle, Joseph A. Capobianco, Myles B. Herbert, Kent S Harvey |
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| Rok vydání: | 2019 |
| Předmět: |
biology
010405 organic chemistry Chemistry General Chemical Engineering Mannose General Chemistry Carbohydrate 010402 general chemistry biology.organism_classification 01 natural sciences Rapid detection Fluorescence 3. Good health 0104 chemical sciences chemistry.chemical_compound Salmonella enterica Biophysics QD1-999 Boronic acid Bacteria Mannan |
| Zdroj: | ACS Central Science, Vol 5, Iss 5, Pp 789-795 (2019) |
| ISSN: | 2374-7951 2374-7943 |
| Popis: | [Image: see text] Reliable early-stage detection of foodborne pathogens is a global public health challenge that requires new and improved sensing strategies. Here, we demonstrate that dynamically reconfigurable fluorescent double emulsions can function as highly responsive optical sensors for the rapid detection of carbohydrates fructose, glucose, mannose, and mannan, which are involved in many biological and pathogenic phenomena. The proposed detection strategy relies on reversible reactions between boronic acid surfactants and carbohydrates at the hydrocarbon/water interface leading to a dynamic reconfiguration of the droplet morphology, which alters the angular distribution of the droplet’s fluorescent light emission. We exploit this unique chemical–morphological–optical coupling to detect Salmonella enterica, a type of bacteria with a well-known binding affinity for mannose. We further demonstrate an oriented immobilization of antibodies at the droplet interface to permit higher selectivity. Our demonstrations yield a new, inexpensive, robust, and generalizable sensing strategy that can help to facilitate the early detection of foodborne pathogens. |
| Databáze: | OpenAIRE |
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