Polysaccharide-based tissue-engineered vascular patches
| Autor: | Fernanda Carla Bombaldi de Souza, Ketul C. Popat, Ângela Maria Moraes, Diego Mantovani, Bernard Drouin, Renata Francielle Bombaldi de Souza |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Scaffold
Materials science food.ingredient Pectin Alginates Polymers Myocytes Smooth Muscle Biocompatible Materials Bioengineering 02 engineering and technology 010402 general chemistry Polysaccharide 01 natural sciences Biomaterials Chitosan chemistry.chemical_compound food Tissue engineering Polysaccharides Humans Vascular Diseases Cells Cultured Vascular tissue chemistry.chemical_classification Tissue Engineering Tissue Scaffolds Regeneration (biology) Polymer 021001 nanoscience & nanotechnology 0104 chemical sciences chemistry Mechanics of Materials Pectins 0210 nano-technology Biomedical engineering |
| Zdroj: | Materials Science and Engineering: C. 104:109973 |
| ISSN: | 0928-4931 |
| DOI: | 10.1016/j.msec.2019.109973 |
| Popis: | Coronary artery and peripheral vascular diseases are the leading cause of morbidity and mortality worldwide and often require surgical intervention to replace damaged blood vessels, including the use of vascular patches in endarterectomy procedures. Tissue engineering approaches can be used to obtain biocompatible and biodegradable materials directed to this application. In this work, dense or porous scaffolds constituted of chitosan (Ch) complexed with alginate (A) or pectin (P) were fabricated and characterized considering their application as tissue-engineered vascular patches. Scaffolds fabricated with alginate presented higher culture medium uptake capacity (up to 17 g/g) than materials produced with pectin. A degradation study of the patches in the presence of lysozyme showed longer-term stability for Ch-P-based scaffolds. Pectin-containing matrices presented higher elastic modulus (around 280 kPa) and ability to withstand larger deformations. Moreover, these materials demonstrated better performance when tested for hemocompatibility, with lower levels of platelet adhesion and activation. Human smooth muscle cells (HSMC) adhered, spread and proliferated better on matrices produced with pectin, probably as a consequence of cell response to higher stiffness of this material. Thus, the outcomes of this study demonstrate that Ch-P-based scaffolds present superior characteristics for the application as vascular patches. Despite polysaccharides are yet underrated in this field, this work shows that biocompatible tridimensional structures based on these polymers present high potential to be applied for the reconstruction and regeneration of vascular tissues. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect