Effect of UV-B (302 nm) irradiation on isolated rat hepatocytes
| Autor: | Subhra Lahiri, Rajeev Naithani, Aleem Ahmed Khan, V. K. Srinivas, Qamer Ayesha, C. M. Habibullah |
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| Rok vydání: | 1995 |
| Předmět: |
Cell Survival
Ultraviolet Rays Biology In Vitro Techniques Radiation Dosage chemistry.chemical_compound Ornithine Carbamoyltransferase Lactate dehydrogenase medicine Animals Urea Irradiation Na+/K+-ATPase Rats Wistar chemistry.chemical_classification Hepatology L-Lactate Dehydrogenase In vitro Rats medicine.anatomical_structure Enzyme chemistry Biochemistry Liver Hepatocyte Sodium-Potassium-Exchanging ATPase |
| Zdroj: | Liver. 15(3) |
| ISSN: | 0106-9543 |
| Popis: | The present study aimed to evaluate the effect of UV-B irradiation on the functional integrity, and the metabolic and detoxifying capacity of isolated rat hepatocytes. Isolated rat hepatocytes were irradiated in various doses (400 Jm-2, 600 Jm-2, 800 Jm-2 and 1000 Jm-2). The cells were assayed for total lactate dehydrogenase, Na(+)-K(+)-ATPase, ornithine carbamyltransferase activity (OCT) and urea production capacity. Lactate dehydrogenase and Na(+)-K(+)-ATPase activity were significantly decreased in all four irradiated groups (P < 0.001), whereas viability, OCT and urea production capacity showed no alterations. |
| Databáze: | OpenAIRE |
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