Haustorium initiation in the obligate parasitic plant Phelipanche ramosa involves a host-exudated cytokinin signal

Autor: Sandra Pelletier, Lukáš Spíchal, Philippe Delavault, Fabrice Monteau, Marc-Marie Lechat, Estelle Billard, Vincent Goyet, Philippe Simier, Jean-Bernard Pouvreau, Grégory Montiel, Muriel Bahut
Přispěvatelé: Université de Nantes (UN), Institut de Recherche en Horticulture et Semences (IRHS), Université d'Angers (UA)-Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS), Palacky University Olomouc, French Ministry of Education and Research, Region Pays de Loire (RFI Objectif Vegetal project), Ministry of Education, Youth and Sports of the Czech Republic (National Program of Sustainability) LO1204
Jazyk: angličtina
Rok vydání: 2017
Předmět:
[SDV.SA]Life Sciences [q-bio]/Agricultural sciences
0106 biological sciences
0301 basic medicine
angiosperme
Cytokinins
genetic structures
Physiology
acide abscisique
Plant Science
01 natural sciences
root exudates
Plant Roots
Phelipanche ramosa
Transcriptome
chemistry.chemical_compound
Plant Growth Regulators
méristème racinaire
Haustorium
heterocyclic compounds
Vegetal Biology
biology
food and beverages
histidine kinase
[SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics
Research Papers
Agricultural sciences
3. Good health
Botanique
haustorium
Cytokinin
gène candidat
Growth and Development
récepteur
microarray
psychological phenomena and processes
Botanics
root development
Phytopathology and phytopharmacy
Parasitic plant
03 medical and health sciences
Botany
[SDV.BV]Life Sciences [q-bio]/Vegetal Biology
Gene
Allelopathy
parasitic plant
Obligate
Brassica napus
broomrape
cytokinins
Host (biology)
Orobanche
fungi
biology.organism_classification
Phytopathologie et phytopharmacie
[SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy
arabidopsis
030104 developmental biology
chemistry
Biologie végétale
Sciences agricoles
010606 plant biology & botany
Zdroj: Journal of Experimental Botany
Journal of Experimental Botany, Oxford University Press (OUP), 2017, 68 (20), pp.5539-5552. ⟨10.1093/jxb/erx359⟩
Journal of Experimental Botany 20 (68), 5539-5552. (2017)
ISSN: 1460-2431
0022-0957
DOI: 10.1093/jxb/erx359⟩
Popis: The formation of the haustorium in holoparasitic plants as demonstrated in Phelipanche ramosa is initiated through perception of a host-exudated cytokinin and a cytokinin signaling response.
The heterotrophic lifestyle of parasitic plants relies on the development of the haustorium, a specific infectious organ required for attachment to host roots. While haustorium development is initiated upon chemodetection of host-derived molecules in hemiparasitic plants, the induction of haustorium formation remains largely unknown in holoparasitic species such as Phelipanche ramosa. This work demonstrates that the root exudates of the host plant Brassica napus contain allelochemicals displaying haustorium-inducing activity on P. ramosa germinating seeds, which increases the parasite aggressiveness. A de novo assembled transcriptome and microarray approach with P. ramosa during early haustorium formation upon treatment with B. napus root exudates allowed the identification of differentially expressed genes involved in hormone signaling. Bioassays using exogenous cytokinins and the specific cytokinin receptor inhibitor PI-55 showed that cytokinins induced haustorium formation and increased parasite aggressiveness. Root exudates triggered the expression of cytokinin-responsive genes during early haustorium development in germinated seeds, and bio-guided UPLC-ESI(+)-/MS/MS analysis showed that these exudates contain a cytokinin with dihydrozeatin characteristics. These results suggest that cytokinins constitutively exudated from host roots play a major role in haustorium formation and aggressiveness in P. ramosa.
Databáze: OpenAIRE
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