Electric fields contribute to directed migration of human retinal pigment epithelial cells via interaction between F-actin and beta1 integrin
| Autor: | Jie Zhu, Jing Han, Yan-Nian Hui, Quan-Hong Han, Xiaolong Yan, Yue Li |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Cell Survival
Integrin Blotting Western Retinal Pigment Epithelium Biology Cellular and Molecular Neuroscience chemistry.chemical_compound Electromagnetic Fields Cell Movement Humans Cytochalasin RNA Messenger Cytoskeleton Fluorescent Antibody Technique Indirect Actin Cells Cultured Messenger RNA Reverse Transcriptase Polymerase Chain Reaction Integrin beta1 Cell migration Retinal Molecular biology Sensory Systems Actins Cell biology Blot Ophthalmology chemistry biology.protein sense organs |
| Zdroj: | Current eye research. 34(6) |
| ISSN: | 1460-2202 |
| Popis: | Purpose: To observe the effects of electric fields (EFs) on the migration of human retinal pigment epithelial (RPE) cells and to explore possible related mechanisms. Methods: Cultured human RPE cells were exposed to EFs, and images of the cells were obtained at regular intervals to evaluate the cell migration and viability. Distribution of F-actin and β 1 integrin was measured by immunohistochemistry. Expression of β 1 integrin was determined by PCR and Western blotting. Results: Exposure to EFs resulted in a cathodal-directed migration of RPE cells and a cathodal accumulation of F-actin and β 1 integrin in the cells. EF stimulation increased expression of β 1 integrin both in mRNA and protein levels. These effects could be inhibited by cytochalasin B. Conclusion: EFs induce directed migration of RPE cells, and this effect is, to some extent, regulated by the interaction between cytoskeleton and integrins. |
| Databáze: | OpenAIRE |
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