Amino Acid Substitution in the Major Multidrug Efflux Transporter Protein AcrB Contributes to Low Susceptibility to Azithromycin in Haemophilus influenzae
| Autor: | Hidemasa Nakaminami, Shoji Seyama, Norihisa Noguchi, Takeaki Wajima |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.drug_class 030106 microbiology Nonsense mutation Microbial Sensitivity Tests Drug resistance Azithromycin medicine.disease_cause Haemophilus influenzae Microbiology Macrolide Antibiotics 03 medical and health sciences Bacterial Proteins Mechanisms of Resistance Drug Resistance Bacterial Operon medicine Pharmacology (medical) Pharmacology Mutation Chemistry Membrane Transport Proteins bacterial infections and mycoses Anti-Bacterial Agents Multiple drug resistance Infectious Diseases Amino Acid Substitution Efflux Multilocus Sequence Typing medicine.drug |
| Zdroj: | Antimicrobial Agents and Chemotherapy. 61 |
| ISSN: | 1098-6596 0066-4804 |
| DOI: | 10.1128/aac.01337-17 |
| Popis: | Clarithromycin-resistant Haemophilus influenzae strains with a nonsense mutation in acrR generally exhibited susceptibility to azithromycin, although one strain was found to be nonsusceptible; we aimed to clarify the differences. This strain had an amino acid substitution, Arg327Ser, in AcrB. Introduction of this substitution into H. influenzae Rd caused an increase in the MIC of azithromycin, suggesting that this substitution contributed to nonsusceptibility. These findings indicate that azithromycin-nonsusceptible isolates could occur through stepwise mutation in the acr region. |
| Databáze: | OpenAIRE |
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