Anaerobic induction in conifers: Expression of endogenous and chimeric anaerobically-induced genes
| Autor: | G. Pilate, Faouzi Bekkaoui, David I. Dunstan, William L. Crosby, Allen G. Good |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
chemistry.chemical_classification
Reporter gene Physiology fungi Heterologous Life Sciences Promoter Cell Biology Plant Science General Medicine Biology chemistry.chemical_compound Enzyme chemistry Biochemistry Cell culture Lactate dehydrogenase Gene expression biology.protein Genetics Alcohol dehydrogenase |
| Zdroj: | Biological Sciences Publications |
| Popis: | Alcohol dehydrogenase (ADH; EC 1.1.1.1) activity was measured in Picea glauca (Moench) Voss cell suspensions under differing conditions of hypoxia. ADH activity increased 4.5 fold after 48 h of induction. When cells were induced under different levels of hypoxia (2, 5 and 20% O2) changes in ADH activity were found to increase with lower levels of oxygen. Alanine aminotransferase (AlaAT; EC 2.6.1.2) activity increased under hypoxia in a pattern similar to ADH, however lactate dehydrogenase (LDH; EC 1.1.1.27) activity did not increase under hypoxic conditions. The ability of white spruce cells to accurately regulate heterologous anaerobic promotors was tested by electroporating chimeric ADH reporter genes into protoplasts. While protoplasts were capable of anaerobically regulating a maize ADH reporter construct, constructs with dicotyledonous promoters (pea and Arabidopsis) were not expressed. |
| Databáze: | OpenAIRE |
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