Real-time quantitative PCR assay with Taqman ® probe for rapid detection of MCR-1 plasmid-mediated colistin resistance
| Autor: | Linda Hadjadj, S. Le Page, Liliane Okdah, Selma Chabou, Thongpan Leangapichart, J.-M. Rolain |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Klebsiella pneumoniae 030106 microbiology Biology medicine.disease_cause Microbiology New Technologies for Infectious and Tropical Diseases lcsh:Infectious and parasitic diseases 03 medical and health sciences chemistry.chemical_compound Plasmid medicine TaqMan lcsh:RC109-216 Antibiotic resistance surveillance Escherichia coli Gene PCR detection Taqman probe biology.organism_classification Molecular biology Infectious Diseases Real-time polymerase chain reaction colistin resistance chemistry mcr-1 gene MCR-1 hormones hormone substitutes and hormone antagonists DNA |
| Zdroj: | New Microbes and New Infections, Vol 13, Iss C, Pp 71-74 (2016) New Microbes and New Infections |
| ISSN: | 2052-2975 |
| DOI: | 10.1016/j.nmni.2016.06.017 |
| Popis: | Here we report the development of two rapid real-time quantitative PCR assays with TaqMan® probes to detect the MCR-1 plasmid-mediated colistin resistance gene from bacterial isolates and faecal samples from chickens. Specificity and sensitivity of the assay were 100% on bacterial isolates including 18 colistin-resistant isolates carrying the mcr-1 gene (six Klebsiella pneumoniae and 12 Escherichia coli) with a calibration curve that was linear from 101 to 108 DNA copies. Five out of 833 faecal samples from chickens from Algeria were positive, from which three E. coli strains were isolated and confirmed to harbour the mcr-1 gene by standard PCR and sequencing. |
| Databáze: | OpenAIRE |
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