A rapid assay for measuring the metabolism of [3H]-retinoic acid in cell cultures
| Autor: | David William End, Thomas A. Garrabrant |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Time Factors
Cytochrome Retinoic acid Mammary Neoplasms Animal Tretinoin Adenocarcinoma Toxicology High-performance liquid chromatography chemistry.chemical_compound Mice Radioligand Assay medicine Tumor Cells Cultured Animals Liarozole Solid phase extraction Chromatography High Pressure Liquid Pharmacology Chromatography biology Clotrimazole Metabolism chemistry Biochemistry biology.protein Ketoconazole Female medicine.drug |
| Zdroj: | Journal of pharmacological and toxicological methods. 34(4) |
| ISSN: | 1056-8719 |
| Popis: | A simple method was developed to detect the metabolism of [3H]-retinoic acid to polar products using intact tumor cells in culture. Unaltered [3H]-retinoic acid was separated from more polar metabolites using C18-bonded solid phase extraction cartridges. Separation of unaltered retinoic acid and polar metabolites was confirmed by HPLC. The murine mammary carcinoma cell line TA3 Ha used in these studies converted 40% to 50% of added radioactive retinoic acid to polar metabolites released into the culture medium during a 4-hr incubation period. Metabolism of [3H]-retinoic acid by TA3 Ha cells was inhibited by the cytochrome P-450 inhibitors ketoconazole, clotrimazole, and liarozole. The simplicity and rapidity of this assay should make it useful for evaluating compounds as inhibitors of retinoic acid metabolism. |
| Databáze: | OpenAIRE |
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