Chemical and morphological effects of the contraceptive hormone 17 ?-ethynylestradiol on fluid lipid membranes
| Autor: | Ludovico Valli, Carlos J. L. Constantino, Gilia Cristine Marques Ruiz, Luis F. C. Morato, Francesco Milano, Wallance Moreira Pazin, Livia Giotta |
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| Přispěvatelé: | Ruiz, G. C. M., do Carmo Morato, L. F., Pazin, W. M., Milano, F., Constantino, C. J. L., Valli, L., Giotta, L., Universidade Estadual Paulista (Unesp), Italian National Research Council (CNR-ISPA), S.P. Lecce-Monteroni, Unità di Lecce |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Langmuir
17α-ethynylestradiol Lipid Bilayers Phospholipid 02 engineering and technology Ethinyl Estradiol POPC 01 natural sciences chemistry.chemical_compound Colloid and Surface Chemistry Contraceptive Agents 0103 physical sciences Monolayer 17?-ethynylestradiol Humans Langmuir monolayer Physical and Theoretical Chemistry Small unilamellar vesicles Giant unilamellar vesicle Phospholipids Unilamellar Liposomes Langmuir monolayers 010304 chemical physics Vesicle technology industry and agriculture Surfaces and Interfaces General Medicine Penetration (firestop) 021001 nanoscience & nanotechnology PM-IRRAS Membrane chemistry Phosphatidylcholines Biophysics lipids (amino acids peptides and proteins) Giant unilamellar vesicles 0210 nano-technology Biotechnology Hormone |
| Zdroj: | Colloids and surfaces. B, Biointerfaces 204 (2021). doi:10.1016/j.colsurfb.2021.111794 info:cnr-pdr/source/autori:Ruiz G.C.M.; do Carmo Morato L.F.; Pazin W.M.; Milano F.; Constantino C.J.L.; Valli L.; Giotta L./titolo:Chemical and morphological effects of the contraceptive hormone 17 ?-ethynylestradiol on fluid lipid membranes/doi:10.1016%2Fj.colsurfb.2021.111794/rivista:Colloids and surfaces. B, Biointerfaces (Print)/anno:2021/pagina_da:/pagina_a:/intervallo_pagine:/volume:204 Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| DOI: | 10.1016/j.colsurfb.2021.111794 |
| Popis: | Made available in DSpace on 2021-06-25T11:15:08Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-08-01 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Instituto Nacional de Ciência e Tecnologia em Eletrônica Orgânica Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) The lack of studies involving the effects in human health associated with the chronic ingestion of pollutants lead to the path of investigating the action of these compounds in cell membrane models. We demonstrated the interaction (causes and consequences) of the hormone 17 α-ethinylestradiol (EE2) with lipid monolayers (prepared as Langmuir films) and bilayers prepared as small unilamellar vesicles (SUVs) and giant unilamellar vesicles (GUVs). Both fluidity and majority chemical composition of real plasma cell membrane were guaranteed using the phospholipid 1-palmitoil-2-oleoyl-sn-glycero-3-phosphatidylcholine (POPC). Surface pressure-mean molecular area (π-A) isotherms and PM-IRRAS measurements highlighted the strong interaction of EE2 with POPC monolayers, leading the hormone to remain at the air/water interface and promoting its penetration into the phospholipid hydrophobic chains. In the case of bilayers, the entrance of the hormone inside the SUV is likely facilitated by their high curvature. In GUVs, EE2 was responsible for changes in the spherical shape, forming structures like buds and lipid protrusions. The set of results indicates the strong effects of EE2 on fluid membranes, which is an important feature to predict its damage in human cells. Department of Physics School of Technology and Applied Sciences São Paulo State University (UNESP) Institute of Sciences of Food Production Italian National Research Council (CNR-ISPA), S.P. Lecce-Monteroni Department of Biological and Environmental Sciences and Technologies (DiSTeBA) University of Salento S.P. Lecce-Monteroni Consorzio Interuniversitario Nazionale per la Scienza e Tecnologia dei Materiali (INSTM) Unità di Lecce, S.P. Lecce-Monteroni Department of Physics School of Technology and Applied Sciences São Paulo State University (UNESP) FAPESP: 2016/09633-4 FAPESP: 2018/222147-6 CAPES: 88882.330129/2018-01 |
| Databáze: | OpenAIRE |
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