An unattended HS-SPME-GC-MS/MS combined with a novel sample preparation strategy for the reliable quantitation of C8 volatiles in mushrooms: A sample preparation strategy to fully control the volatile emission
| Autor: | Sun Hye Baek, Mun Yhung Jung, Seung-Hyun Kim, Jae-Gu Han, Ill-Min Chung, Da Eun Lee, Su Min Lim |
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| Rok vydání: | 2020 |
| Předmět: |
Shiitake Mushrooms
Mass spectrometry 01 natural sciences Gas Chromatography-Mass Spectrometry Analytical Chemistry chemistry.chemical_compound 0404 agricultural biotechnology Limit of Detection Tandem Mass Spectrometry Sample preparation Solid Phase Microextraction Mushroom Principal Component Analysis Volatile Organic Compounds Chromatography Chemistry 010401 analytical chemistry 04 agricultural and veterinary sciences General Medicine 040401 food science 0104 chemical sciences Flavoring Agents Gas chromatography Gas chromatography–mass spectrometry Citric acid Agaricales Food Science Shiitake mushrooms |
| Zdroj: | Food chemistry. 347 |
| ISSN: | 1873-7072 |
| Popis: | Eight carbon (C8) compounds are the key characteristic flavors of mushrooms. The quantitative analysis of the volatiles in mushrooms is challenging especially with the unattended HS-SPME-GC-MS. An unattended HS-SPME-GC-MS/MS in combination with novel sample preparation of the complete control of volatile emissions was developed for the quantitation of the C8 volatiles in mushrooms. The sample preparation strategy was composed of freeze-drying, rehydration, and the addition of a 15% citric acid solution. With this strategy, the volatile emission from mushroom was fully controlled at a certain time point. This method was found to be highly reliable, sensitive, precise, and accurate. This method was successfully applied to measure the contents of the C8 volatiles in the beech, button, and shiitake mushrooms. 1-Octene-3-ol was the most predominant compound in the mushrooms, representing 62.4, 69.0, and 89.2% of the total C8 volatiles in the beech, button, and shiitake mushrooms, respectively. |
| Databáze: | OpenAIRE |
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