General mechanism for the bacterial toxicity of hypochlorous acid: abolition of ATP production
| Autor: | James K. Hurst, William C. Barrette, Diane M. Hannum, William D. Wheeler |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
Hypochlorous acid
Adenylate kinase Oxidative phosphorylation medicine.disease_cause Biochemistry Microbiology chemistry.chemical_compound Adenosine Triphosphate Biosynthesis medicine Escherichia coli chemistry.chemical_classification ATP synthase biology Biological Transport Succinates Hypochlorous Acid Lactococcus lactis Proton-Translocating ATPases Enzyme Glucose chemistry Fermentation Pseudomonas aeruginosa biology.protein Energy source Energy Metabolism |
| Zdroj: | Biochemistry. 28(23) |
| ISSN: | 0006-2960 |
| Popis: | The adenylate energy charges (EC) of Escherichia coli 25922, Pseudomonas aeruginosa 27853, and Streptococcus lactis 7962 rapidly fell in nutrient-rich media from values in excess of 0.9 to below 0.1 when the organisms were exposed to lethal levels of HOCl. The same cells maintained in energy-depleted states were incapable of attaining normal EC values necessary for biosynthesis and growth when challenged with nutrient energy sources after HOCl exposure. These changes correlated quantitatively with loss of replicative capabilities. Initial rates of transport of glucose, succinate, and various amino acids that act as respiratory substrates and the ATP hydrolase activity of the F1 complex from the ATP synthase of E. coli 25922 also declined in parallel with or preceded loss of viability. These results establish that cellular death is accompanied by complete disruption of bacterial ATP production by both oxidative and fermentative pathways as a consequence of inhibition of inner membrane bound systems responsible for these processes. |
| Databáze: | OpenAIRE |
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