Coupling of Termination, 3′ Processing, and mRNA Export

Autor: Claire Moore, Christopher M. Hammell, Charles N. Cole, Catherine V. Heath, Daniel Zenklusen, Françoise Stutz, Stefan Gross
Rok vydání: 2002
Předmět:
Transcription
Genetic
RNA
Messenger/metabolism
Polynucleotide Adenylyltransferase/metabolism
Receptors
Cytoplasmic and Nuclear
Pancreatitis-Associated Proteins
RNA-binding protein
RNA polymerase II
RNA/metabolism
Transcription (biology)
Cell and Organelle Structure and Assembly
Recombination
Genetic
biology
Temperature
Nuclear Proteins
Polynucleotide Adenylyltransferase
RNA-Binding Proteins
Flow Cytometry
Precursor mRNA
Saccharomyces cerevisiae Proteins
Green Fluorescent Proteins
Saccharomyces cerevisiae/genetics/metabolism/physiology
Saccharomyces cerevisiae
Karyopherins
Fungal Proteins
Open Reading Frames
ddc:570
Two-Hybrid System Techniques
Polyadenylate
RNA
Messenger
Fungal Proteins/metabolism
Molecular Biology
Gene
Alleles
Nuclear Proteins/metabolism
MRNA Cleavage and Polyadenylation Factors
Cell Nucleus
mRNA Cleavage and Polyadenylation Factors
Messenger RNA
Biological Transport
Cell Biology
Molecular biology
Luminescent Proteins/metabolism
Luminescent Proteins
Open reading frame
Cell Nucleus/metabolism
Karyopherins/metabolism
Mutation
biology.protein
RNA
RNA-Binding Proteins/metabolism
Gene Deletion
Zdroj: Molecular and Cellular Biology, Vol. 22, No 18 (2002) pp. 6441-57
ISSN: 1098-5549
0270-7306
DOI: 10.1128/mcb.22.18.6441-6457.2002
Popis: In a screen to identify genes required for mRNA export in Saccharomyces cerevisiae, we isolated an allele of poly(A) polymerase (PAP1) and novel alleles encoding several other 3' processing factors. Many newly isolated and some previously described mutants (rna14-48, rna14-49, rna14-64, rna15-58, and pcf11-1 strains) are defective in polymerase II (Pol II) termination but, interestingly, retain the ability to polyadenylate these improperly processed transcripts at the nonpermissive temperature. Deletion of the cis-acting sequences required to couple 3' processing and termination also produces transcripts that fail to exit the nucleus, suggesting that all of these processes (cleavage, termination, and export) are coupled. We also find that several but not all mRNA export mutants produce improperly 3' processed transcripts at the nonpermissive temperature. 3' maturation defects in mRNA export mutants include improper Pol II termination and/or the previously characterized hyperpolyadenylation of transcripts. Importantly, not all mRNA export mutants have defects in 3' processing. The similarity of the phenotypes of some mRNA export mutants and 3' processing mutants indicates that some factors from each process may mechanistically interact to couple mRNA processing and export. Consistent with this assumption, we present evidence that Xpo1p interacts in vivo with several 3' processing factors and that the addition of recombinant Xpo1p to in vitro processing reaction mixtures stimulates 3' maturation. Of the core 3' processing factors tested (Rna14p, Rna15p, Pcf11p, Hrp1p, Fip1p, and Cft1p), only Hrp1p shuttles. Overexpression of Rat8p/Dbp5p suppresses both 3' processing and mRNA export defects found in xpo1-1 cells.
Databáze: OpenAIRE
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