Developmental control of IFN-alpha expression in murine embryos
| Autor: | Paulette Duc-Goiran, Italina Cerutti, Ahmet Civas, Martine Maury, Janine Doly, Sébastien Navarro, Brigitte Robert, Hubert Condamine, Christiane Rudant |
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| Rok vydání: | 1994 |
| Předmět: |
Cellular differentiation
Placenta Molecular Sequence Data In situ hybridization Biology Polymerase Chain Reaction Mice Transcription (biology) Pregnancy Image Processing Computer-Assisted Animals Tissue Distribution Northern blot RNA Messenger In Situ Hybridization Caliciviridae Infections Messenger RNA Fetus Base Sequence RNA Interferon-alpha Embryo Cell Biology Blotting Northern Embryo Mammalian Molecular biology Norwalk virus Animals Newborn Autoradiography Female DNA Probes |
| Zdroj: | Experimental cell research. 214(2) |
| ISSN: | 0014-4827 |
| Popis: | The expression of IFN-alpha transcripts was investigated in murine embryos, fetuses, and fetal annexes in mid and late pregnancy. We have shown by Northern blot analysis, reverse transcription-polymerase chain reaction, and in situ hybridization the presence of IFN-alpha transcripts in mouse placenta, fetus, and newborn. From the 14th day of gestation until birth, a typical IFN-alpha transcript (1.2 kb) is found in the fetus. A transcript of larger size (2.2 kb) appears near birth and is present in the newborn mouse. Fetal annexes between the 10th and 21st days of gestation also express IFN-alpha. From the 10th day until birth, the 1.2-kb IFN-alpha mRNA species is present, as well as unusually large transcripts: 4 and 7 kb. To localize IFN-alpha transcripts, in situ hybridization was performed, using 35S-IFN-alpha antisense RNA probe in comparison with the sense RNA probe. The tissue pattern of IFN-alpha transcription in fetuses shows a clear labeling of many epithelia, such as skin, ependyme, and intestine glandular epithelium. A possible relation with cellular differentiation is discussed. |
| Databáze: | OpenAIRE |
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