Simultaneous determination of glycyl–l-histidyl–l-lysine and its metabolite, l-histidyl–l-lysine, in rat plasma by high-performance liquid chromatography with post-column derivatization
| Autor: | Masaharu Miyagi, Arao Ujiie, Takuro Endo |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Male
Detection limit Chromatography Metabolite Lysine Reproducibility of Results Dipeptides General Chemistry Tripeptide Sensitivity and Specificity High-performance liquid chromatography Rats chemistry.chemical_compound O-Phthalaldehyde Drug Stability chemistry Animals Growth Substances Derivatization Oligopeptides Quantitative analysis (chemistry) Chromatography High Pressure Liquid o-Phthalaldehyde |
| Zdroj: | Journal of Chromatography B: Biomedical Sciences and Applications. 692:37-42 |
| ISSN: | 0378-4347 |
| DOI: | 10.1016/s0378-4347(96)00460-4 |
| Popis: | A selective and sensitive high-performance liquid chromatographic (HPLC) method was developed for the determination of glycyl-L-histidyl-L-lysine (GHK), a liver-cell growth factor isolated from human plasma, and its metabolite, L-histidyl-L-lysine (HK), in rat plasma. Both high selectivity and sensitivity were achieved by the use of solid-phase extraction with a Bond-Elut Certify cartridge, ion-pair chromatography with 1-pentanesulfonate on a 5-microm Capcell Pak C18 UG120 column (250x4.6 mm I.D.) with a guard column, and by post-column derivatization with o-phthalaldehyde (OPA). GHK and HK were extracted from 0.1 ml of rat plasma after addition of o-phenanthroline to protect against degradation. The limit of detection for GHK and HK were 50 and 15 ng/ml, respectively, and the calibration curves were linear in the range 0.1-5.0 microg/ml. The developed method was applied to the pharmacokinetic study of GHK after a single dose was administered intravenously to rats. GHK was rapidly degraded to HK, which was eliminated rapidly. |
| Databáze: | OpenAIRE |
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