Investigation of calcium crystals in OA knees
| Autor: | Christoph H. Lohmann, Joern Steinhagen, Wolfgang Rüther, Martin Fuerst, Oliver Niggemeyer, F. K. W. Schäfer, L. Lammers |
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| Rok vydání: | 2009 |
| Předmět: |
Calcium Phosphates
Cartilage Articular Male musculoskeletal diseases Adolescent Knee Joint Immunology Chondrocalcinosis Osteoarthritis Matrix (biology) Calcium Pyrophosphate Spectrum Analysis Raman Mineralization (biology) Calcium Carbonate Effective algorithm Young Adult Chondrocytes Nuclear magnetic resonance Rheumatology Chondrocyte cell medicine Humans Immunology and Allergy Arthroplasty Replacement Knee Cells Cultured Aged business.industry Cartilage Anatomy Osteoarthritis Knee medicine.disease Calcium crystals Radiographic Image Enhancement Durapatite medicine.anatomical_structure Cell culture Case-Control Studies Microscopy Electron Scanning Female Crystallization business Algorithms |
| Zdroj: | Rheumatology International. 30:623-631 |
| ISSN: | 1437-160X 0172-8172 |
| Popis: | For studies on matrix mineralization in osteoarthritis (OA), a clear analytical approach is necessary to identify and to quantify mineralization in the articular cartilage. The aim of this study is to develop an effective algorithm to quantify and to identify cartilage mineralization in the experimental setting. Four patients with OA of the knee undergoing total knee replacement and four control patients were included. Cartilage calcification was studied by digital contact radiography (DCR), field emission scanning electron microscopy (FE-SEM) X-ray element analysis and Raman spectroscopy (RS). DCR revealed mineralization in all OA cartilage specimens. No mineralization was observed in the control cartilage. Patient I showed rhomboid shaped crystals with a mean Ca:P molar ratio of 1.04 indicated the presence of calcium pyrophosphate dihydrate (CPPD) crystals, while Patients II, III and IV presented carbonate-substituted hydroxyapatite (HA). RS also showed the presence of CPPD crystals in Patient I while Patients II, III and IV revealed spectra confirming the presence of HA crystals. In the corresponding chondrocyte cell culture analyzed with SEM, the presence of CPPD crystals in the culture of Patient I and HA crystals in the culture of Patient II, III and IV was confirmed. No mineralization was found in the cell culture of the controls. The differentiation between BCP and CPPD crystals plays an important role, and the techniques presented here provide an accurate differentiation of these two types of crystals. For quantification of articular cartilage mineralization, DCR is a simple and accurate method. |
| Databáze: | OpenAIRE |
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