The sodium iodide symporter is unlikely to be a thyroid/breast shared antigen
| Autor: | Lei Zhang, Ilaria Muller, Claudio Giani, Fiona Grennan-Jones, Marian Ludgate, Colin M. Dayan |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Adult
Male Sodium-iodide symporter endocrine system medicine.medical_specialty Adolescent Endocrinology Diabetes and Metabolism Population Breast Neoplasms 030209 endocrinology & metabolism CHO Cells Autoantigens Iodide Peroxidase Flow cytometry Young Adult 03 medical and health sciences Cricetulus 0302 clinical medicine Endocrinology Antigen Cricetinae Iron-Binding Proteins Internal medicine medicine Animals Humans Lactoperoxidase education health care economics and organizations Aged Aged 80 and over education.field_of_study Symporters medicine.diagnostic_test Chemistry Thyroid Thyroiditis Autoimmune Autoantibody Antibodies Monoclonal Middle Aged Flow Cytometry Thyroid Diseases Molecular biology medicine.anatomical_structure 030220 oncology & carcinogenesis Symporter Female |
| Zdroj: | Journal of Endocrinological Investigation. 39:323-331 |
| ISSN: | 1720-8386 |
| DOI: | 10.1007/s40618-015-0368-6 |
| Popis: | Anti-thyroid peroxidase (TPO) autoantibodies (TPOAb) seem to be protective for patients with breast cancer (BC). Thyroid and breast tissues both express the sodium iodide symporter (NIS), similarly both have a peroxidase activity, TPO and lactoperoxidase (LPO) respectively. We hypothesize a common immune response to a thyroid/breast shared antigen suggesting three putative mechanisms: (1) TPOAb react to both TPO and LPO, (2) TPO could be expressed in BC and (3) patients with TPOAb could have autoantibodies to NIS (NISAb). Previous studies excluded NISAb that block NIS activity in sera of patients with thyroid autoimmunity (TA) and/or BC. This study investigates neutral NISAb (binding without affecting function). Clones of CHO cells stably expressing human NIS (hNIS; CHO-NIS) were isolated following transfection of hNIS in pcDNA3 vector. Expression of hNIS mRNA and surface protein was confirmed by PCR and flow cytometry respectively using a hNIS-mouse-monoclonal-antibody. CHO-NIS and controls transfected with the empty pcDNA3 vector (CHO-Empty) were incubated with 42 heat-inactivated human sera followed by an anti-human-IgG-AlexaFluor488-conjugate: 12 with BC, 11 with TA, 10 with both BC and TA and 9 with non-autoimmune thyroid diseases. The Kolmogorov–Smirnov Test was used to compare the fluorescence intensity obtained with CHO-NIS and CHO-Empty, using sera from six young males as a negative control population. None of the 42 sera were positive for NISAb. NISAb are rare and NIS is unlikely to be a common thyroid/BC shared antigen. We have recently demonstrated TPO expression in BC tissue and are currently investigating TPOAb cross-reactivity with TPO/LPO. |
| Databáze: | OpenAIRE |
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