Identification of strain diversity and phylogenetic analysis based on two major essential proteins of Orf viruses isolated from several clinical cases reported in Malaysia
| Autor: | Muhammad Syaafii bin Noorzahari, Mohd Termizi Ghazali, Ramlan Bin Mohamed, Ashwaq Ahmed Abdullah, Abd Wahid Haron, Krishnan Nair Balakrishnan, Faez Firdaus Abdullah Jesse, M. L. Mohd-Azmi, Mustapha Mohamed Noordin, Jamilu Abubakar Bala |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Orf virus viruses 030106 microbiology Sheep Diseases Molecular Technique Biology Microbiology 03 medical and health sciences symbols.namesake Viral Proteins Microscopy Electron Transmission Genotype Chlorocebus aethiops Genetics Contagious ecthyma Ecthyma Contagious Animals Molecular Biology Vero Cells Ecology Evolution Behavior and Systematics Phylogeny Sanger sequencing Goat Diseases Sheep Phylogenetic tree Strain (biology) Goats Malaysia Genetic Variation Sequence Analysis DNA Virology 030104 developmental biology Infectious Diseases Vero cell symbols |
| Zdroj: | Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases. 77 |
| ISSN: | 1567-7257 |
| Popis: | There is a little information on the characterization of Orf virus strains that are endemic in Malaysia. The relationship between the severity of disease and the molecular genetic profile of Orf virus strains has not been fully elucidated. This study documented the first confirmed report of contagious ecthyma causing by Orf virus in goats from a selected state of eastern peninsular Malaysia. The disease causes significant debilitation due to the inability of affected animals to suckle which brings a great economic loss to the farmers. A total of 504 animals were examined individually to recognize the affected animals with Orf lesion. Skin scrapping was used to collect the scab material from the infected animals. The presence of Orf virus was confirmed by combination of methods including virus isolation on vero cells, identification by Transmission Electron Microscopy (TEM) and molecular technique using PCR and Sanger sequencing. The results showed the successful isolation of four Orf virus strains with a typical cytopathic effects on the cultured vero cells line. The morphology was confirmed to be Orf virus with a distinctive ovoid and criss cross structure. The phylogenetic analysis revealed that these isolated strains were closely related to each other and to other previously isolated Malaysian orf viruses. In addition these Orf virus strains were closely related to Orf viruses from China and India. This study provides more valuable insight in terms of genotype of Orf virus circulating in Malaysia. |
| Databáze: | OpenAIRE |
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