Liposome/DNA systems: correlation between association, hydrophobicity and cell viability
| Autor: | Silvia del Valle Alonso, Luciia Speroni, Maria C. Taira, Nadia Silvia Chiaramoni |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Cell Survival
Bioengineering Applied Microbiology and Biotechnology chemistry.chemical_compound Phosphatidylcholine Chlorocebus aethiops Animals Ammonium Viability assay Vero Cells Unilamellar Liposomes Liposome Chromatography Chemistry Cholesterol technology industry and agriculture Cationic polymerization Gene Transfer Techniques General Medicine DNA Membrane lipids (amino acids peptides and proteins) Hydrophobic and Hydrophilic Interactions Biotechnology Plasmids |
| Zdroj: | Biotechnology letters. 29(11) |
| ISSN: | 0141-5492 |
| Popis: | Small unilamellar vesicles associated with plasmid DNA showed maximum association efficiency for a cationic mixture of egg phosphatidylcholine (EPC):1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE):di-1,2-dioleoyl-3-trimethyl ammonium propane (DOTAP) (16:8:1 molar ratio) [65%], followed by neutral lipids EPC:1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine (DMPE):cholesterol (Chol) (2:2:1 molar ratio) [30%], and a polymerized formulation 1,2-bis(10,12-tricosadiynoyl)sn-glycero-3-phosphocholine (DC8,9PC):DMPE:Chol (2:2:1 molar ratio) [11%]. The hydrophobicity factor (HF) for these formulations followed the trend DC8,9PC:DMPE:CHOL < EPC:DMPE:Chol < EPC:DOPE DOTAP, and DNA association did not alter this trend. Results suggest that the higher the HF value, the more fluid the membrane and the higher the efficiency of DNA association. On the other hand, no differences were observed in cell toxicity with lipids up to 1 mg/ml in VERO cells. |
| Databáze: | OpenAIRE |
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