Dynamics of cytokine mRNA expression and fecal biomarkers in school-children undergoing a double-blind placebo-controlled food challenge series
| Autor: | Ignacio Arencibia, Anna Winberg, Olga Nagaeva, Catarina Lundell, Ivan Nagaev, Eva Rönmark, Lucia Mincheva-Nilsson, Christina E. West |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male medicine.medical_treatment Immunology Placebo Biochemistry 03 medical and health sciences Feces 0302 clinical medicine Double-Blind Method Food allergy Immunology and Allergy Medicine Humans RNA Messenger Child Molecular Biology business.industry Hematology medicine.disease Interleukin 10 030104 developmental biology Cytokine 030228 respiratory system Interleukin 13 Cytokines Cytokine mrna Female Calprotectin business Leukocyte L1 Antigen Complex Biomarkers Food Hypersensitivity |
| Zdroj: | Cytokine. 88 |
| ISSN: | 1096-0023 |
| Popis: | There is need for prognostic markers for symptomatic food allergy since current diagnostic methods are insufficient and/or time and labor consuming.To estimate the cytokine mRNA profiles in peripheral blood mononuclear cells (PBMC) before and after a double-blind placebo-controlled food challenge series in schoolchildren with suspected allergy to milk, egg or cod and in healthy controls. Analyses of fecal inflammatory biomarkers before and after the challenge were included.Twelve-year-old children from a population-based cohort reporting complete avoidance of milk, egg, cod or wheat due to perceived hypersensitivity were clinically examined and those with suspected food allergy were evaluated with a 3-session double-blind placebo-controlled food challenge (n=18). Seven healthy controls participated in a double-blind challenge with egg. Before and after the challenge series, the cytokine mRNA expression was quantified for 13 cytokines discriminating between humoral Th2-, cytotoxic Th1-, regulatory Th3/Tr1- and inflammatory responses. Fecal calprotectin and eosinophil-derived neurotoxin (EDN) were also analyzed in children with suspected food allergy before and after the challenge series.Pre challenge, children with suspected food allergy had higher IL-13andTNF-α expression and lower IFN-γ and IL-15 expression compared to healthy controls (all p0.05). Children with challenge-proven food allergy had increased IL13andIL-10 expression compared to the levels seen in negative challenges (p0.05). Post challenge, IL-1β and IL-6 mRNA levels were elevated in the food allergic children compared to controls (p0.05). Fecal calprotectin and EDN levels were higher in challenge-proven food allergy compared to a negative challenge although not statistically significantly.Increased baseline mRNA levels of the Th2-related cytokine IL-13 and the regulatory cytokine IL-10 predicted a positive food challenge outcome. These cytokines in combination with fecal calprotectin and EDN might serve as future prognostic markers for symptomatic, IgE-mediated food allergy but need further validation in a larger patient cohort. |
| Databáze: | OpenAIRE |
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