Analysis with a Combination of Macrorestriction Endonucleases Reveals a High Degree of Polymorphism among Bordetella pertussis Isolates in Eastern France
| Autor: | F. I. S. Freitas, P. Stoessel, Jean-Michel Scheftel, O. Meunier, M. Haubensack, Gilles Prévost, H. Monteil |
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| Rok vydání: | 1999 |
| Předmět: |
DNA
Bacterial Male Microbiology (medical) Bordetella pertussis Whooping Cough Disease Outbreaks Ribotyping Genotype Pulsed-field gel electrophoresis medicine Humans Typing Child Whooping cough Genetics Molecular Epidemiology Polymorphism Genetic Molecular epidemiology biology Infant Bacteriology DNA Restriction Enzymes biology.organism_classification medicine.disease DNA Fingerprinting Bacterial Typing Techniques Electrophoresis Gel Pulsed-Field DNA profiling Child Preschool Female France Polymorphism Restriction Fragment Length |
| Zdroj: | Journal of Clinical Microbiology. 37:1062-1068 |
| ISSN: | 1098-660X 0095-1137 |
| Popis: | From 1990 to 1996, routine screening for whooping cough identified 399 patients with a calmodulin-dependent adenylate cyclase-positive test result and yielded 69 Bordetella pertussis isolates. None of the patients were fully vaccinated, and most were less than 6 months old. Analysis of total DNA by pulsed-field gel electrophoresis (PFGE) after Xba I, Spe I, or Dra I macrorestriction yielded 19, 15, and 5 different patterns, respectively, whereas ribotyping failed to demonstrate any strain polymorphism. Discrimination among the isolates was improved by combining the PFGE profiles. Some patterns were more frequent, but the corresponding patients were not clearly epidemiologically related. The patterns for two strains obtained during a 3-month period from patients who were neighbors differed by the length of a single DNA fragment. These data strongly suggest that one type of isolate is widely spread throughout the world and is carried by individuals other than patients who develop a true illness. |
| Databáze: | OpenAIRE |
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