Consistency of staining and reporting of oestrogen receptor immunocytochemistry within the European Union—an inter-laboratory study

Autor: Fritz Rank, Janina Kulka, Thomas Decker, A. Reiner, Vincenzo Eusebi, Peter Regitnig, Gábor Cserni, Isabel Amendoeira, N Apostolikas, Manuela Lacerda, A.M. Tanous, S. Thorstenson, E. Saksela, C Munt, J. Martinez-Penuela, Clive A. Wells, Bettina Borisch, Giovanni Bussolati, Maria Drijkoningen, Simonetta Bianchi, D Coleman, Päivi Heikkilä, Johannes L. Peterse, John P. Sloane, C. de Miguel, Brigitte Sigal-Zafrani, C.E. Connolly, D Faverly, Jocelyne Jacquemier, Peter A. Dervan, Ian O. Ellis, Anna Sapino, C. W. Elston, Jean-Pierre Bellocq, Roland Holland, M Sylvan, Werner Boecker, E. Zozaya, H. Kerner
Přispěvatelé: Wells CA, Sloane JP, Coleman D, Munt C, Amendoeira I, Apostolikas N, Bellocq JP, Bianchi S, Boecker W, Bussolati G, Connolly CE, Dervan P, Drijkoningen M, Ellis IO, Elston CW, Eusebi V, Faverly D, Heikkila P, Holland R, Jacqemier J, Lacerda M, Martinez-Penuela J, De Miguel C, Peterse JL, Rank F, Saksela E, Sigal-Zafrani B, Sylvan M, Borisch B, Cserni G, Decker T, Kerner H, Kulka J, Regitnig P, Sapino A, Tanous AM, Thorstenson S, Zozaya E.
Rok vydání: 2004
Předmět:
Zdroj: Virchows Archiv, 445, 119-28
Virchows Archiv, 445, 2, pp. 119-28
ISSN: 1432-2307
0945-6317
DOI: 10.1007/s00428-004-1063-8
Popis: Contains fulltext : 58820.pdf (Publisher’s version ) (Closed access) To assess the variability of oestrogen receptor (ER) testing using immunocytochemistry, centrally stained and unstained slides from breast cancers were circulated to the members of the European Working Group for Breast Screening Pathology, who were asked to report on both slides. The results showed that there was almost complete concordance among readers (kappa=0.95) in ER-negative tumours on the stained slide and excellent concordance among readers (kappa=0.82) on the slides stained in each individual laboratory. Tumours showing strong positivity were reasonably well assessed (kappa=0.57 and 0.4, respectively), but there was less concordance in tumours with moderate and low levels of ER, especially when these were heterogeneous in their staining. Because of the variation, the Working Group recommends that laboratories performing these stains should take part in a external quality assurance scheme for immunocytochemistry, should include a tumour with low ER levels as a weak positive control and should audit the percentage positive tumours in their laboratory against the accepted norms annually. The Quick score method of receptor assessment may also have too many categories for good concordance, and grouping of these into fewer categories may remove some of the variation among laboratories.
Databáze: OpenAIRE
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