Quantitative multi-target RNA profiling in Epstein-Barr virus infected tumor cells
| Autor: | Octavia Ramayanti, Astrid E. Greijer, Hedy Juwana, Jaap M. Middeldorp, Sandra A. W. M. Verkuijlen, Zlata Novalić |
|---|---|
| Přispěvatelé: | Pathology, AII - Cancer immunology, CCA - Cancer immunology |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Herpesvirus 4 Human medicine.medical_treatment Cell Biology Virus Replication medicine.disease_cause Virus Targeted therapy Viral Matrix Proteins Viral Proteins 03 medical and health sciences 0302 clinical medicine hemic and lymphatic diseases Virology Tumor Cells Cultured medicine Humans RNA Messenger Gene Expression Profiling RNA Epstein–Barr virus Virus Latency 030104 developmental biology medicine.anatomical_structure Epstein-Barr Virus Nuclear Antigens Lytic cycle Viral replication 030220 oncology & carcinogenesis RNA Viral Carcinogenesis Biomarkers |
| Zdroj: | Greijer, A E, Ramayanti, O, Verkuijlen, S A W M, Novalić, Z, Juwana, H & Middeldorp, J M 2016, ' Quantitative multi-target RNA profiling in Epstein-Barr virus infected tumor cells ', Journal of Virological Methods, vol. 241, pp. 24-33 . https://doi.org/10.1016/j.jviromet.2016.12.007 Journal of Virological Methods, 241, 24-33. Elsevier |
| ISSN: | 0166-0934 |
| DOI: | 10.1016/j.jviromet.2016.12.007 |
| Popis: | Epstein-Barr virus (EBV) is etiologically linked to multiple acute, chronic and malignant diseases. Detection of EBV-RNA transcripts in tissues or biofluids besides EBV-DNA can help in diagnosing EBV related syndromes. Sensitive EBV transcription profiling yields new insights on its pathogenic role and may be useful for monitoring virus targeted therapy. Here we describe a multi-gene quantitative RT-PCR profiling method that simultaneously detects a broad spectrum (n=16) of crucial latent and lytic EBV transcripts. These transcripts include (but are not restricted to), EBNA1, EBNA2, LMP1, LMP2, BARTs, EBER1, BARF1 and ZEBRA, Rta, BGLF4 (PK), BXLF1 (TK) and BFRF3 (VCAp18) all of which have been implicated in EBV-driven oncogenesis and viral replication. With this method we determine the amount of RNA copies per infected (tumor) cell in bulk populations of various origin. While we confirm the expected RNA profiles within classic EBV latency programs, this sensitive quantitative approach revealed the presence of rare cells undergoing lytic replication. Inducing lytic replication in EBV tumor cells supports apoptosis and is considered as therapeutic approach to treat EBV-driven malignancies. This sensitive multi-primed quantitative RT-PCR approach can provide broader understanding of transcriptional activity in latent and lytic EBV infection and is suitable for monitoring virus-specific therapy responses in patients with EBV associated cancers. |
| Databáze: | OpenAIRE |
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