Restriction endonuclease analysis of Aspergillus fumigatus DNA
| Autor: | J P Burnie, A P Coke, R C Matthews |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
EcoRI
Biology Genome Pathology and Forensic Medicine Microbiology Aspergillus fumigatus chemistry.chemical_compound Endonuclease Aspergillosis Humans DNA Fungal skin and connective tissue diseases Electrophoresis Agar Gel General Medicine biology.organism_classification DNA Fingerprinting Molecular biology Restriction enzyme chemistry DNA profiling biology.protein Genome Fungal Caesium chloride Polymorphism Restriction Fragment Length DNA Research Article |
| Zdroj: | Journal of Clinical Pathology. 45:324-327 |
| ISSN: | 0021-9746 |
| DOI: | 10.1136/jcp.45.4.324 |
| Popis: | AIMS: To develop a genome based DNA fingerprinting system for Aspergillus fumigatus mould. METHODS: DNA was extracted from 21 isolates obtained from eight patients with an aspergilloma. This was with a freeze-dried mycelial extract fragmented in liquid nitrogen. DNA was subsequently purified by phenol-chloroform extraction followed by ultracentrifugation on a caesium chloride gradient. The DNA was restricted by EcoRI and Xba I. RESULTS: All isolates were identical when cut by EcoRI; Xba I delineated six DNA types. CONCLUSIONS: DNA fingerprinting can be used to type isolates of A fumigatus. Strains from within an aspergilloma which were morphologically distinct could either have the identical DNA fingerprint or produce a unique type. |
| Databáze: | OpenAIRE |
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