Analytical accuracy of determinations of aminoglycoside concentrations by enzyme multiplied immunoassay, fluorescence polarization immunoassay, and radioimmunoassay in the presence of heparin
| Autor: | Gary R. Matzke, Charles E. Halstenson, M E O'Connell, K L Heim |
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| Jazyk: | angličtina |
| Rok vydání: | 1984 |
| Předmět: |
Microbiology (medical)
Enzyme multiplied immunoassay technique Chromatography medicine.diagnostic_test Chemistry Heparin Aminoglycoside Radioimmunoassay Fluorescence Polarization equipment and supplies Anti-Bacterial Agents carbohydrates (lipids) Immunoenzyme Techniques Aminoglycosides Immunoassay Immunology medicine Tobramycin Humans Gentamicin Netilmicin medicine.drug Research Article |
| Popis: | The accuracy of gentamicin, netilmicin, and tobramycin concentration determinations by enzyme multiplied immunoassay technique (EMIT; Syva Corp., Palo Alto, Calif.), fluorescence polarization immunoassay (TDx; Abbott Diagnostics, Irving, Tex.), and radioimmunoassay were compared in the presence of 0 to 3,000 USP units of porcine heparin per ml. Gentamicin, netilmicin, and tobramycin concentrations determined by EMIT decreased by 10 and 50% in the presence of 75 and 1,000 USP units/ml, 2 and 5 USP units/ml, and 2 and 7.5 USP units/ml, respectively. Accuracy of the TDx and radioimmunoassay determinations, however, were not affected by the presence of heparin. Blood samples for the determination of gentamicin, netilmicin, and tobramycin by EMIT should not be collected in evacuated heparinized tubes. |
| Databáze: | OpenAIRE |
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