Loss of UBE3A from TH-expressing neurons suppresses GABA co-release and enhances VTA-NAc optical self-stimulation
| Autor: | Marie Rougie, Zoe A. McElligott, Pranish A. Kantak, Benjamin D. Philpot, Garret D. Stuber, Megumi Aita, Janet Berrios, Alice M. Stamatakis, Matthew C. Judson |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Patch-Clamp Techniques Dopamine General Physics and Astronomy Synaptic Transmission Nucleus Accumbens Stereotaxic Techniques Mice 0302 clinical medicine Self Stimulation Neural Pathways In Situ Hybridization Fluorescence gamma-Aminobutyric Acid Mice Knockout Neurons Multidisciplinary Behavior Animal Dopaminergic Glutamate receptor Immunohistochemistry Ventral tegmental area medicine.anatomical_structure Biochemistry Reinforcement Psychology medicine.drug Tyrosine 3-Monooxygenase Science Ubiquitin-Protein Ligases Neurotransmission Biology Nucleus accumbens General Biochemistry Genetics and Molecular Biology gamma-Aminobutyric acid Article 03 medical and health sciences Reward medicine Animals Motivation Dopaminergic Neurons Ventral Tegmental Area General Chemistry Mice Inbred C57BL Optogenetics 030104 developmental biology nervous system Stereotaxic technique Neuroscience 030217 neurology & neurosurgery |
| Zdroj: | Nature Communications Nature Communications, Vol 7, Iss 1, Pp 1-8 (2016) |
| ISSN: | 2041-1723 |
| Popis: | Motivated reward-seeking behaviours are governed by dopaminergic ventral tegmental area projections to the nucleus accumbens. In addition to dopamine, these mesoaccumbal terminals co-release other neurotransmitters including glutamate and GABA, whose roles in regulating motivated behaviours are currently being investigated. Here we demonstrate that loss of the E3-ubiquitin ligase, UBE3A, from tyrosine hydroxylase-expressing neurons impairs mesoaccumbal, non-canonical GABA co-release and enhances reward-seeking behaviour measured by optical self-stimulation. Mesoaccumbal terminals within the VTA are known to co-release both GABA and dopamine, although the functional role of the former has yet to be determined. Here, the authors find that non-canonical GABA release is regulated by the E3-ubiquitin ligase, UBE3A, and enhances optogenetic self-stimulation. |
| Databáze: | OpenAIRE |
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