Peripheral Neuronopathy Associated With Ebola Virus Infection in Rhesus Macaques: A Possible Cause of Neurological Signs and Symptoms in Human Ebola Patients
| Autor: | Amanda M.W. Hischak, Louis Huzella, John G. Bernbaum, Richard S. Bennett, Donna L. Perry, David X. Liu, Timothy K. Cooper, Randy Hart, Lisa E. Hensley |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male Sympathetic nervous system Pathology medicine.medical_specialty Necrosis Sympathetic Nervous System Sensory Receptor Cells viruses Antigens Differentiation Myelomonocytic Disease In situ hybridization medicine.disease_cause 03 medical and health sciences Major Articles and Brief Reports 0302 clinical medicine Dorsal root ganglion Antigens CD Parasympathetic Nervous System Ganglia Spinal medicine Immunology and Allergy Animals Humans 030212 general & internal medicine Ganglion Cysts Ebola virus business.industry CD68 Macrophages Peripheral Nervous System Diseases Hemorrhagic Fever Ebola Ebolavirus Immunohistochemistry Macaca mulatta Disease Models Animal 030104 developmental biology Infectious Diseases medicine.anatomical_structure Peripheral nervous system Nerve Degeneration Leukocytes Mononuclear Female Ganglia Microglia medicine.symptom business |
| Zdroj: | J Infect Dis |
| Popis: | Neurological signs and symptoms are the most common complications of Ebola virus disease. However, the mechanisms underlying the neurologic manifestations in Ebola patients are not known. In this study, peripheral ganglia were collected from 12 rhesus macaques that succumbed to Ebola virus (EBOV) disease from 5 to 8 days post exposure. Ganglionitis, characterized by neuronal degeneration, necrosis, and mononuclear leukocyte infiltrates, was observed in the dorsal root, autonomic, and enteric ganglia. By immunohistochemistry, RNAscope in situ hybridization, transmission electron microscopy, and confocal microscopy, we confirmed that CD68+ macrophages are the target cells for EBOV in affected ganglia. Further, we demonstrated that EBOV can induce satellite cell and neuronal apoptosis and microglial activation in infected ganglia. Our results demonstrate that EBOV can infect peripheral ganglia and results in ganglionopathy in rhesus macaques, which may contribute to the neurological signs and symptoms observed in acute and convalescent Ebola virus disease in human patients. |
| Databáze: | OpenAIRE |
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