Hormone- and endotoxin-modulated gene expression of a long-term organ culture system of adult rat liver
| Autor: | Ernesto G. Bade, J. Hartung, K. Sultan |
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| Rok vydání: | 1996 |
| Předmět: |
Lipopolysaccharides
Male medicine.medical_specialty Immunoblotting Nitric oxide synthase repression Biophysics Cycloheximide Organ culture Biochemistry Dexamethasone Gene Expression Regulation Enzymologic chemistry.chemical_compound Organ Culture Techniques Tyrosine aminotransferase Endotoxin Structural Biology Long-term organ culture Internal medicine Gene expression Genetics medicine Animals Enzyme inducer Glucocorticoids Molecular Biology Tyrosine Transaminase Regulation of gene expression Tyrosine aminotransferase induction biology Cell Biology Rats Endocrinology Liver chemistry Rats Inbred Lew Enzyme Induction biology.protein Enzyme Repression Nitric Oxide Synthase Precision-cut liver slice Glucocorticoid Hormone medicine.drug |
| Zdroj: | FEBS Letters. 394:51-54 |
| ISSN: | 0014-5793 |
| Popis: | Precision-cut slices of normal adult rat liver maintained in serum-free medium remain hormone- and endotoxin-responsive for at least 48 h. They respond to glucocorticoid (dexamethasone) with the induction of the gluconeogenic enzyme tyrosine aminotransferase (TAT), as determined by enzymatic activity and by the increase in enzyme protein. Furthermore, endotoxin (LPS) induced nitric oxide synthase II (i-NOS), and this induction is repressed, similarly to the in vivo situation, by dexamethasone (DEX). All increases are inhibited by cycloheximide (CHX). The length of the period of responsiveness suggests that this organ culture system might be generally useful for studying the modulation of liver gene expression by physiological and pathological influences. |
| Databáze: | OpenAIRE |
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