Effect of antiviral treatment of chronic hepatitis C on the frequency of regulatory T cells, T-cell activation, and serum levels of TGF-beta
| Autor: | Jaroslav Kapla, Pavel Bostik, Ondřej Beran, Simona Arientová, Alžběta Davidová, Michal Holub, Stanislav Plíšek, Kateřina Kondělková, Pavel Chalupa |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Male
Serum 0301 basic medicine Hepacivirus CD8-Positive T-Lymphocytes CD38 Lymphocyte Activation medicine.disease_cause T-Lymphocytes Regulatory 0302 clinical medicine Immunophenotyping Transforming Growth Factor beta Interferon Immunology and Allergy Membrane Glycoproteins biology General Medicine Hepatitis C Middle Aged Flow Cytometry medicine.anatomical_structure 030220 oncology & carcinogenesis Female medicine.drug Adult Microbiology (medical) Genotype T cell Hepatitis C virus Antiviral Agents Pathology and Forensic Medicine Young Adult 03 medical and health sciences Ribavirin medicine Humans Protease Inhibitors Aged business.industry Interferon-alpha HLA-DR Antigens Transforming growth factor beta Hepatitis C Chronic medicine.disease ADP-ribosyl Cyclase 1 030104 developmental biology Immunology biology.protein business CD8 |
| Zdroj: | APMIS. 124:711-718 |
| ISSN: | 0903-4641 |
| DOI: | 10.1111/apm.12561 |
| Popis: | The aim was to analyze T-regulatory cells (Tregs), activated CD8(+) T cells, and transforming growth factor-beta (TGF)-β in hepatitis C patients. We enrolled 31 patients with chronic genotype 1 hepatitis C virus (HCV) infection, 30 seropositive persons with spontaneous HCV elimination, and 23 healthy volunteers. The patients were examined at the beginning of the interferon-alpha (IFN-α)-based therapy (baseline) and at weeks 4 (W4) and 12 (W12) of the therapy. The percentage of Tregs and the expression of activation markers CD38 and HLA-DR on CD8(+) T cells were analyzed in the peripheral blood by flow cytometry. Serum levels of TGF-β were measured in a multiplex assay using flow cytometry. The percentage of Tregs in patients was higher than in controls and seropositive persons. Similarly, the percentage of CD8(+) T cells expressing CD38 and HLA-DR was higher in patients compared with controls and seropositive persons. Chronic HCV infection is associated with elevated circulating Tregs and activated CD8(+) T cells. During IFN-α-based therapy these cells gradually increase, whereas TGF-β serum levels decrease. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|