Enhanced neogenic potential of Panc-1 cells supplemented with human umbilical cord blood serum--An alternative to FCS
| Autor: | Ramesh R. Bhonde, S. S. Challa, G. Sashi Kiran, Vijayalakshmi Venkatesan |
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| Rok vydání: | 2011 |
| Předmět: |
Serum
Cell Culture Techniques Biology Umbilical cord Andrology Islets of Langerhans Blood serum Cell Line Tumor medicine Humans Cell Proliferation Fetus geography geography.geographical_feature_category Mesenchymal stem cell Cell Differentiation Cell Biology General Medicine Islet Fetal Blood Staining Culture Media Transplantation medicine.anatomical_structure Cell culture Immunology lipids (amino acids peptides and proteins) Developmental Biology |
| Zdroj: | Tissuecell. 43(4) |
| ISSN: | 1532-3072 |
| Popis: | The promise(s) of using Fetal Calf Serum (FCS) as a supplement for the maintenance of cell cultures has been well documented. However, FCS forms the xenogenic source for any human derived cells/organ and limits its application. Recently, the usage of human umbilical cord blood serum (hUCBS) for maintenance of mesenchymal cells has been supportive. In the present study we investigated the effects of hUCBS and FCS on the proliferation (viability, proliferative) and its differentiation potential (DTZ staining, immunofluroscence) to generate islet like cellular aggregates (ICAs) using the human derived Panc-1 cell lines. A comparative analysis of hUCBS and FCS for each parameter demonstrated that hUCBS supplemented media was better for proliferation and differentiation of the Panc-1 cells. The ICAs obtained from hUCBS primed cultures showed a higher yield, increased islet size, and showed an increase for insulin staining compared to FCS. We suggest that hUCBS can be explored as an alternate serum supplement for FCS, making it more feasible in cell systems of human derived origin and can also find its application for the human transplantation programmes. |
| Databáze: | OpenAIRE |
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