Thein vitroantileishmanial activity of essential oil fromAloysia gratissimaand guaiol, its major sesquiterpene againstLeishmania amazonensis
| Autor: | Deivid Costa Soares, Antonio Carlos Siani, Maria das Graças Miranda Danelli, Elvira M. Saraiva, Raissa Couto Santana, Lucia H. Pinto-da-Silva, M.C.F. Garcia, Thaïs Souto-Padrón, Mônica Freiman de Souza Ramos |
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| Rok vydání: | 2018 |
| Předmět: |
030231 tropical medicine
Antiprotozoal Agents Biology Sesquiterpene 01 natural sciences law.invention Microbiology Inhibitory Concentration 50 Mice Sesquiterpenes Guaiane 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Cutaneous leishmaniasis law Oils Volatile medicine Animals Amastigote IC50 Cells Cultured Essential oil Leishmania Life Cycle Stages Mice Inbred BALB C Plant Extracts Guaiol medicine.disease biology.organism_classification 0104 chemical sciences 010404 medicinal & biomolecular chemistry Infectious Diseases chemistry Kinetoplast Macrophages Peritoneal Protozoa Animal Science and Zoology Parasitology Sesquiterpenes |
| Zdroj: | Parasitology. 145:1219-1227 |
| ISSN: | 1469-8161 0031-1820 |
| DOI: | 10.1017/s0031182017002335 |
| Popis: | Leishmaniases is a tropical disease caused by protozoa of the genusLeishmaniafor which the current treatment is expensive, besides increasing reports of parasite resistance. This study investigated the anti-Leishmania amazonensisactivity of the essential oil fromAloysia gratissima(AgEO) and guaiol, the major sesquiterpene constituent in the oil. Our results showed that AgEO killed promastigotes and intracellular amastigotes at an IC50of 25 and 0·16µg mL−1, respectively, while guaiol killed amastigotes at an IC50of 0·01µg mL−1. Both AgEO and guaiol were safe for macrophages up to 100µg mL−1, as evaluated by the dehydrogenase activity, membrane integrity and phagocytic capacity. AgEO and guaiol did not induce nitrite oxide (NO) in resting macrophages and inhibited the production of NO in lipopolysaccharide-stimulated macrophages. The ultrastructural analysis suggested that AgEO and guaiol act directly on parasites, affecting promastigotes kinetoplast, mitochondrial matrix and plasma membrane. Together, these results pointed out that AgEO and guaiol could be promising candidates to develop anti-Leishmaniadrugs. |
| Databáze: | OpenAIRE |
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