Prevalence and Characteristics of Phenicol-Oxazolidinone Resistance Genes in
| Autor: | Min Hyeok Cha, Seung-Min Yang, Gun Jo Woo, Hae-Yeong Kim, So Won Shin, Yoon Soo Gwak, Eiseul Kim, Hyo Sun Kwak |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Swine
Enterococcus faecium Genome Enterococcus fexA Plasmid Anti-Infective Agents Drug Resistance Multiple Bacterial Enterococcus faecalis Biology (General) antibiotic resistance gene Spectroscopy optrA poxtA General Medicine Computer Science Applications Chemistry Horizontal gene transfer Plasmids Transposable element Meat Gene Transfer Horizontal QH301-705.5 linezolid Biology Article Catalysis Microbiology Inorganic Chemistry Republic of Korea Animals Physical and Theoretical Chemistry QD1-999 Molecular Biology Gene Oxazolidinones Whole Genome Sequencing phenicol-oxazolidinone resistance gene Organic Chemistry Computational Biology biology.organism_classification Chloramphenicol Genes Bacterial Cattle Food Analysis Genome Bacterial Multilocus Sequence Typing |
| Zdroj: | International Journal of Molecular Sciences, Vol 22, Iss 11335, p 11335 (2021) International Journal of Molecular Sciences Volume 22 Issue 21 |
| ISSN: | 1422-0067 |
| Popis: | The use of phenicol antibiotics in animals has increased. In recent years, it has been reported that the transferable gene mediates phenicol-oxazolidinone resistance. This study analyzed the prevalence and characteristics of phenicol-oxazolidinone resistance genes in Enterococcus faecalis and Enterococcus faecium isolated from food-producing animals and meat in Korea in 2018. Furthermore, for the first time, we reported the genome sequence of E. faecalis strain, which possesses the phenicol-oxazolidinone resistance gene on both the chromosome and plasmid. Among the 327 isolates, optrA, poxtA, and fexA genes were found in 15 (4.6%), 8 (2.5%), and 17 isolates (5.2%), respectively. Twenty E. faecalis strains carrying resistance genes belonged to eight sequence types (STs), and transferability was found in 17 isolates. The genome sequences revealed that resistant genes were present in the chromosome or plasmid, or both. In strains EFS17 and EFS108, optrA was located downstream of the ermA and ant(9)-1 genes. The strains EFS36 and EFS108 harboring poxtA-encoding plasmid cocarried fexA and cfr(D). These islands also contained IS1216E or the transposon Tn554, enabling the horizontal transfer of the phenicol-oxazolidinone resistance with other antimicrobial-resistant genes. Our results suggest that it is necessary to promote the prudent use of antibiotics through continuous monitoring and reevaluation. |
| Databáze: | OpenAIRE |
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