Interspecific polymorphism of gene lipL32 restriction profiles of pathogenic leptospires
Autor: | O. A. Freilikhman, A. N. Vaganova, N. A. Stoyanova, N. K. Tokarevich |
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Rok vydání: | 2010 |
Předmět: |
Genetics
Leptospira Lipoproteins food and beverages General Medicine Biology Molecular biology General Biochemistry Genetics and Molecular Biology Terminal restriction fragment length polymorphism Restriction enzyme Polymorphism (computer science) Cleaved amplified polymorphic sequence Amplified fragment length polymorphism Restriction fragment length polymorphism Leptospira interrogans Genotyping Gene Polymorphism Restriction Fragment Length Bacterial Outer Membrane Proteins |
Zdroj: | Bulletin of experimental biology and medicine. 149(5) |
ISSN: | 1573-8221 |
Popis: | We analyzed restriction fragment length polymorphism of lipL32 gene encoding outer membrane protein in three leprospira genomic species (L. interrogans, L. kirschneri, and L. borgpetersenii) using Bsa29 I and Bam HI restriction endonucleases. It was found that restriction profiles of the studied gene were similar at both the intraspecific and serogroup levels; variability was revealed only at the interspecific level, which can be explained by relatively low variability of lipL32 gene. |
Databáze: | OpenAIRE |
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