Targeted vertical cross-sectional imaging with handheld near-infrared dual axes confocal fluorescence endomicroscope
| Autor: | Xiyu Duan, Zhen Qiu, Zhongyao Liu, Michael J. Mandella, Bishnu P. Joshi, Kenn R. Oldham, Thomas D. Wang, Supang Khondee, Katsuo Kurabayashi |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
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ocis:(170.5810) Scanning microscopy Materials science Microscope Confocal ocis:(170.4580) Optical diagnostics for medicine 02 engineering and technology Lead zirconate titanate 01 natural sciences law.invention 010309 optics chemistry.chemical_compound Optics Optical coherence tomography law Confocal microscopy 0103 physical sciences medicine Microscopy medicine.diagnostic_test business.industry Near-infrared spectroscopy 021001 nanoscience & nanotechnology Atomic and Molecular Physics and Optics ocis:(170.1790) Confocal microscopy chemistry ocis:(170.3880) Medical and biological imaging 0210 nano-technology business Preclinical imaging Biotechnology |
| Zdroj: | Biomedical Optics Express |
| ISSN: | 2156-7085 |
| Popis: | We demonstrate vertical cross-sectional (XZ-plane) images of near-infrared (NIR) fluorescence with a handheld dual axes confocal endomicroscope that reveals specific binding of a Cy5.5-labeled peptide to pre-malignant colonic mucosa. This view is perpendicular to the tissue surface, and is similar to that used by pathologists. The scan head is 10 mm in outer diameter (OD), and integrates a one dimensional (1-D) microelectromechanical systems (MEMS) X-axis scanner and a bulky lead zirconate titanate (PZT) based Z-axis actuator. The microscope images in a raster-scanning pattern with a ±6 degrees (mechanical) scan angle at ~3 kHz in the X-axis (fast) and up to 10 Hz (0–400 μm) in the Z-axis (slow). Vertical cross-sectional fluorescence images are collected with a transverse and axial resolution of 4 and 5 μm, respectively, over a field-of-view of 800 μm (width) × 400 μm (depth). NIR vertical cross-sectional fluorescence images of fresh mouse colonic mucosa demonstrate histology-like imaging performance with this miniature instrument. |
| Databáze: | OpenAIRE |
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