Heat-directed suicide gene therapy for breast cancer
| Autor: | Paul Szmitko, Duc Ngo, Fei-Fei Liu, Henry J. Klamut, Anthony Brade |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Ganciclovir
Hyperthermia Cancer Research Recombinant Fusion Proteins Apoptosis Breast Neoplasms Biology Thymidine Kinase Adenoviridae Cytosine Deaminase Fusion gene Breast cancer Cell Line Tumor medicine Humans Prodrugs Molecular Biology Reporter gene Cytosine deaminase Genetic Therapy Hyperthermia Induced Suicide gene medicine.disease Combined Modality Therapy Molecular biology Cancer cell Cancer research Molecular Medicine Female medicine.drug |
| Zdroj: | Cancer Gene Therapy. 10:294-301 |
| ISSN: | 1476-5500 0929-1903 |
| DOI: | 10.1038/sj.cgt.7700570 |
| Popis: | Adjuvant hyperthermia can improve treatment outcome for locally recurrent breast cancer (LRBC). Previously, we demonstrated that infection of human breast cancer cells with a recombinant adenovirus expressing beta-galactosidase from the human hsp70b gene promoter (Ad.70b.betagal) results in 50- to 800-fold increases in reporter gene expression following heat treatment (30 minutes at 43 degrees C). Here, we describe a heat-directed suicide gene therapy strategy based on an adenoviral vector (Ad.70b.CDTK) in which expression of the dual prodrug-activating E. coli cytosine deaminase/herpes simplex virus thymidine kinase (CDTK) fusion gene is under the control of the hsp70b promoter. Treatment of T47D and MCF-7 breast cancer cells with mild hyperthermia (43 degrees C/30 minutes) and prodrugs (100 microg/ml 5-fluorocytosine and 10 microg/ml ganciclovir) following infection with Ad.70b.CDTK (10-100 PFU/cell) resulted in 30- to 60-fold decreases in clonogenic survival relative to control cultures treated with heat or prodrugs alone. Clonogenic survival declined even further (up to 240-fold) following heat treatment at 41.5 degrees C for 120 minutes. A decreased clonogenic survival was accompanied by tumor cell apoptosis. These results demonstrate that this combined treatment strategy can be highly effective against heat- and radiation-resistant breast tumor cells and supports the continued development of heat-directed CDTK suicide gene therapy strategies for LRBC. |
| Databáze: | OpenAIRE |
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