Lipid-Oriented Live-Cell Distinction of B and T Lymphocytes
| Autor: | Young-Tae Chang, Stuart N. Berry, Jae Won Lee, Justin Kok Soon Tan, Yun-Kyu Choi, Kangkyun Baek, Solip Park, Jae-Seong Yang, Gun Tae Jung, Kwang Pyo Kim, Soohyun Park, H. W. Lee, Haw-Young Kwon, Sangho Kim, Ki Hean Kim, Raj Kumar Das, Kyeng Min Park, Kimoon Kim, Sun Hyeok Lee, Nam-Young Kang |
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| Přispěvatelé: | National Research Foundation of Korea, Government of South Korea, Institute for Basic Science (South Korea), Pohang University of Science and Technology, Ministry of Education (South Korea) |
| Rok vydání: | 2021 |
| Předmět: |
Cell type
T-Lymphocytes Cell Bone Marrow Cells 010402 general chemistry 01 natural sciences Biochemistry Catalysis Cell membrane Mice Colloid and Surface Chemistry medicine Animals Progenitor cell Fluorescent Dyes B-Lymphocytes biology Chemistry Cell Membrane Lymphocyte differentiation Cell Differentiation General Chemistry Complex cell Flow Cytometry 0104 chemical sciences Cell biology Multicellular organism medicine.anatomical_structure Lipidomics biology.protein Antibody |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 1520-5126 0002-7863 |
| Popis: | The identification of each cell type is essential for understanding multicellular communities. Antibodies set as biomarkers have been the main toolbox for cell-type recognition, and chemical probes are emerging surrogates. Herein we report the first small-molecule probe, CDgB, to discriminate B lymphocytes from T lymphocytes, which was previously impossible without the help of antibodies. Through the study of the origin of cell specificity, we discovered an unexpected novel mechanism of membrane-oriented live-cell distinction. B cells maintain higher flexibility in their cell membrane than T cells and accumulate the lipid-like probe CDgB more preferably. Because B and T cells share common ancestors, we tracked the cell membrane changes of the progenitor cells and disclosed the dynamic reorganization of the membrane properties over the lymphocyte differentiation progress. This study casts an orthogonal strategy for the small-molecule cell identifier and enriches the toolbox for live-cell distinction from complex cell communities. This research was supported by the Institute for Basic Science (IBS) (IBS-R007-A1), the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (no. 2020R1A2C2009776), the Ministry of Education (no. 2020R1A6A1A03047902), and the intramural fund of POSTECH (Pohang University of Science and Technology). |
| Databáze: | OpenAIRE |
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