The influence of acoustic and static stimuli on development of inner ear sensory epithelia
| Autor: | Boris Kablar, Irena Rot |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Cell type
Tyrosine 3-Monooxygenase Cell Count Biology Mice 03 medical and health sciences S100 Calcium Binding Protein G 0302 clinical medicine Developmental Neuroscience Physical Stimulation otorhinolaryngologic diseases medicine Animals Inner ear Muscle Skeletal MyoD Protein 030304 developmental biology Mice Knockout Vestibular system Analysis of Variance 0303 health sciences Hair Cells Auditory Inner Cell Differentiation Anatomy Immunohistochemistry Crista medicine.anatomical_structure Organ of Corti Calbindin 2 Ear Inner Acetylcholinesterase Middle ear MYF5 Myogenic Regulatory Factor 5 sense organs Hair cell 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | International Journal of Developmental Neuroscience. 28:309-315 |
| ISSN: | 1873-474X 0736-5748 |
| Popis: | Mechanical stimuli affect differentiation of specific cell types in several organs of mouse fetuses that develop without any skeletal musculature. To that end, we employed Myf5(-/-):MyoD(-/-) mouse embryos that completely lack skeletal musculature, and analyzed the development of sensory fields in the inner ear. Amyogenic fetuses lack skeletal muscles that move the chain of three middle ear ossicles which normally transfers sound vibrations. They also cannot tilt their head, which prevents the perception of angular acceleration. While our findings in the spiral organ of Corti are surprisingly normal, our results show that the development of cristae ampullaris, vestibular sensory fields sensitive to the angular acceleration, was the most affected. In cristae, hair cells and supporting cells were significantly smaller in the mutant embryos, but hair cells completely lacked tenascin, while supporting cells were more numerous. In maculae, supporting cells were significantly smaller but more numerous in the mutants. Here, we propose that our finding of a specific type I hair cell absence in the mutant's crista may now be employed in the identification of a profile of genes specific for the lacking cell type. |
| Databáze: | OpenAIRE |
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