Humoral and cellular immune responses in mice after airway administration of Bacillus thuringiensis Cry1Ab and MON810 cry1Ab-transgenic maize
| Autor: | Odd Gunnar Wikmark, Unni Cecilie Nygaard, Monica Andreassen, Martinus Løvik, Elena Rocca, Thomas Bøhn, Terje Traavik, Johnnie van der Berg |
|---|---|
| Přispěvatelé: | 12319724 - Van den Berg, Johann |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
medicine.medical_treatment
Immunology Bacillus thuringiensis VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Generell mikrobiologi: 472 VDP::Midical sciences: 700::Basic medical dental and veterinary sciences: 710::Medical immunology: 716 VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Generell immunologi: 478 VDP::Medisinske fag: 700::Basale medisinske odontologiske og veterinærmedisinske fag: 710::Medisinsk immunologi: 716 Immunoglobulin E MON810 Microbiology Immune system medicine Midical sciences: 700::Basic medical dental and veterinary sciences: 710::Medical immunology: 716 [VDP] mouse models VDP::Mathematics and natural science: 400::Basic biosciences: 470::General immunology: 478 Cry1Ab Genetically modified maize biology Immunogenicity fungi food and beverages VDP::Mathematics and natural science: 400::Basic biosciences: 470::General microbiology: 472 biology.organism_classification Genetically modified organism airway allergy Cytokine Medisinske fag: 700::Basale medisinske odontologiske og veterinærmedisinske fag: 710::Medisinsk immunologi: 716 [VDP] biology.protein IgE Antibody Agronomy and Crop Science Food Science |
| Zdroj: | Food and Agricultural Immunology |
| Popis: | Genetically modified (GM) crops may bring new proteins with immunogenic and allergenic properties into the food and feed chains. The most commonly grown GM maize, MON810 expresses a modified version of the insecticidal Cry1Ab protein originating in the soil bacterium Bacillus thuringiensis (Bt). Immune reactions following inhalation of pollen and debris from such plants have been scarcely studied. We exposed BALB/c mice to purified Cry1Ab proteins and Cry1Ab-containing MON810 plant materials by intranasal installation. No anti-Cry1Ab antibodies were detected following exposure to the plant materials. Exposure to purified Cry1Ab resulted in specific anti-Cry1Ab IgG1 and IgE production, indicating inherent immunogenicity and allergenicity. Mice exposed to leaf extracts from both MON810 and unmodified maize demonstrated influx of lymphocytes and eosinophils in the broncho-alveolar lavage, and increased cytokine release in mediastinal lymph node cells. The results indicate that the airway exposure to Cry1Ab proteins may be a route of practical relevance. © 2014 Taylor & Francis. This is the authors* accepted and refereed manuscript to the article. |
| Databáze: | OpenAIRE |
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