Influence of the route of administration on immunomodulatory properties of bovine β-lactoglobulin-producing Lactobacillus casei

Autor: J.-M. Wal, S. Ah-Leung, Stéphane Hazebrouck, Laetitia Przybylski-Nicaise, Gérard Corthier, P. Langella, Karine Adel-Patient
Přispěvatelé: Unité de Recherche Immuno-Allergie Alimentaire, Institut National de la Recherche Agronomique (INRA), Unité de recherche d'Écologie et Physiologie du Système Digestif (UEPSD)
Rok vydání: 2009
Předmět:
Lactobacillus casei
mice
[SDV]Life Sciences [q-bio]
medicine.medical_treatment
Administration
Oral
Lactoglobulins
Immunoglobulin E
Microbiology
Feces
beta lactoglobulin
03 medical and health sciences
Blood serum
Oral administration
Lactobacillus
medicine
Animals
Immunologic Factors
Administration
Intranasal
Sensitization
030304 developmental biology
intranasal and oral application
Mice
Inbred BALB C
0303 health sciences
General Veterinary
General Immunology and Microbiology
biology
030306 microbiology
Public Health
Environmental and Occupational Health
food and beverages
biology.organism_classification
Immunoglobulin A
3. Good health
Lacticaseibacillus casei
Infectious Diseases
medicine.anatomical_structure
Cytokine
Desensitization
Immunologic
Immunoglobulin G
biology.protein
Cytokines
Molecular Medicine
Cattle
Female
Nasal administration
recombinant allergen
Zdroj: Vaccine
Vaccine, Elsevier, 2009, 27 (42), pp.5800-5805. ⟨10.1016/j.vaccine.2009.07.064⟩
ISSN: 0264-410X
DOI: 10.1016/j.vaccine.2009.07.064
Popis: International audience; Because of their intrinsic immunomodulatory properties, some lactic acid bacteria were reported to modulate allergic immune responses in mice and humans. We recently developed recombinant strains of Lactobacillus casei that produce beta-lactoglobulin (BLG), a major cow's milk allergen. Here, we investigated immunomodulatory potency of intranasal and oral administrations of recombinant lactobacilli on a subsequent sensitization of mice to BLG. Intranasal administration of the BLG-producing Lb. casei stimulated serum BLG-specific IgG2a and IgG1 responses, and fecal IgA response as well, but did not inhibit BLG-specific IgE production. In contrast, oral administration led to a significant inhibition of BLG-specific IgE production while IgG1 and IgG2a responses were not stimulated. After both oral and intranasal administrations, production of IL-17 cytokine by BLG-reactivated splenocytes was similarly enhanced, thus confirming the adjuvant effect of the Lb. casei strain. However, a mixed Th1/Th2 cell response was evidenced in BLG-reactivated splenocytes from mice intranasally pretreated, with enhanced secretions of Th1 cytokines (IFN-gamma and IL-12) and Th2 cytokines (IL-4 and IL-5) whereas only production of Th1 cytokines, but not Th2 cytokines, was enhanced in BLG-reactivated splenocytes from mice orally pretreated. Our results show that the mode of administration of live bacteria may be critical for their immunomodulatory effects.
Databáze: OpenAIRE
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