Asymmetric mechanosensitivity in a eukaryotic ion channel
| Autor: | Elisabeth P. Carpenter, Stephen J. Tucker, Viwan Jarerattanachat, Michael V. Clausen, Mark S.P. Sansom |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
K2p channel Multidisciplinary Mechanosensation Cell Membrane Lipid Bilayers Nanotechnology Biology Mechanotransduction Cellular Membrane tension 03 medical and health sciences 030104 developmental biology Membrane Potassium Channels Tandem Pore Domain PNAS Plus Biophysics Humans Mechanosensitive channels Functional studies Lipid bilayer Ion Channel Gating Ion channel |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America. 114(40) |
| ISSN: | 1091-6490 |
| Popis: | Living organisms perceive and respond to a diverse range of mechanical stimuli. A variety of mechanosensitive ion channels have evolved to facilitate these responses, but the molecular mechanisms underlying their exquisite sensitivity to different forces within the membrane remains unclear. TREK-2 is a mammalian two-pore domain (K2P) K+ channel important for mechanosensation, and recent studies have shown how increased membrane tension favors a more expanded conformation of the channel within the membrane. These channels respond to a complex range of mechanical stimuli, however, and it is uncertain how differences in tension between the inner and outer leaflets of the membrane contribute to this process. To examine this, we have combined computational approaches with functional studies of oppositely oriented single channels within the same lipid bilayer. Our results reveal how the asymmetric structure of TREK-2 allows it to distinguish a broad profile of forces within the membrane, and illustrate the mechanisms that eukaryotic mechanosensitive ion channels may use to detect and fine-tune their responses to different mechanical stimuli. |
| Databáze: | OpenAIRE |
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