Antibody against Extracellular Vaccinia Virus (EV) Protects Mice through Complement and Fc Receptors
| Autor: | Gary H. Cohen, Stuart N. Isaacs, Yuhong Xiao, Roselyn J. Eisenberg, Matthew E. Cohen |
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| Rok vydání: | 2011 |
| Předmět: |
Male
lcsh:Medicine Receptors Fc Antibodies Viral Neutralization Immunoglobulin G Mice chemistry.chemical_compound 0302 clinical medicine Emerging Viral Diseases lcsh:Science 0303 health sciences Multidisciplinary Vaccination Opsonin Proteins Immunizations 3. Good health Antibody opsonization Female Antibody Research Article medicine.drug_class Immunology Immunoglobulins Vaccinia virus Biology Monoclonal antibody Microbiology Viral Proteins 03 medical and health sciences Adjuvants Immunologic Virology medicine Animals Immunity to Infections 030304 developmental biology lcsh:R Immunity Immunization Passive Viral Vaccines Complement System Proteins Antibodies Neutralizing Mice Inbred C57BL Animal Models of Infection chemistry Polyclonal antibodies Humoral Immunity biology.protein lcsh:Q CpG Islands Vaccinia Extracellular Space 030215 immunology |
| Zdroj: | PLoS ONE PLoS ONE, Vol 6, Iss 6, p e20597 (2011) |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0020597 |
| Popis: | Protein-based subunit smallpox vaccines have shown their potential as effective alternatives to live virus vaccines in animal model challenge studies. We vaccinated mice with combinations of three different vaccinia virus (VACV) proteins (A33, B5, L1) and examined how the combined antibody responses to these proteins cooperate to effectively neutralize the extracellular virus (EV) infectious form of VACV. Antibodies against these targets were generated in the presence or absence of CpG adjuvant so that Th1-biased antibody responses could be compared to Th2-biased responses to the proteins with aluminum hydroxide alone, specifically with interest in looking at the ability of anti-B5 and anti-A33 polyclonal antibodies (pAb) to utilize complement-mediated neutralization in vitro. We found that neutralization of EV by anti-A33 or anti-B5 pAb can be enhanced in the presence of complement if Th1-biased antibody (IgG2a) is generated. Mechanistic differences found for complement-mediated neutralization showed that anti-A33 antibodies likely result in virolysis, while anti-B5 antibodies with complement can neutralize by opsonization (coating). In vivo studies found that mice lacking the C3 protein of complement were less protected than wild-type mice after passive transfer of anti-B5 pAb or vaccination with B5. Passive transfer of anti-B5 pAb or monoclonal antibody into mice lacking Fc receptors (FcRs) found that FcRs were also important in mediating protection. These results demonstrate that both complement and FcRs are important effector mechanisms for antibody-mediated protection from VACV challenge in mice. |
| Databáze: | OpenAIRE |
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